Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2638161..2639749 Replicon chromosome
Accession NZ_CP045988
Organism Klebsiella pneumoniae strain WCHKP35

Toxin (Protein)


Gene name hipA Uniprot ID A0A486MZ73
Locus tag CEA74_RS12850 Protein ID WP_009485244.1
Coordinates 2638161..2639483 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag CEA74_RS12855 Protein ID WP_002904790.1
Coordinates 2639483..2639749 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CEA74_RS12835 (2633217) 2633217..2635718 + 2502 WP_009485242.1 fimbria/pilus outer membrane usher protein -
CEA74_RS12840 (2635706) 2635706..2636710 + 1005 WP_009485243.1 fimbrial protein -
CEA74_RS12845 (2636852) 2636852..2638144 + 1293 WP_002904794.1 glycoside hydrolase family 10 protein -
CEA74_RS12850 (2638161) 2638161..2639483 - 1323 WP_009485244.1 type II toxin-antitoxin system HipA family toxin Toxin
CEA74_RS12855 (2639483) 2639483..2639749 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
CEA74_RS12860 (2640015) 2640015..2640341 - 327 WP_009485245.1 hydroxyisourate hydrolase -
CEA74_RS12865 (2640338) 2640338..2640838 - 501 WP_002904787.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
CEA74_RS12870 (2640816) 2640816..2642195 - 1380 WP_004184021.1 nucleobase:cation symporter-2 family protein -
CEA74_RS12875 (2642541) 2642541..2643695 + 1155 WP_009485247.1 FAD-dependent urate hydroxylase HpxO -
CEA74_RS12880 (2643676) 2643676..2644599 - 924 WP_002904781.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - mgtC / iroE 2441225..2682116 240891


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA, N-terminal subdomain 1

(4-103)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49157.50 Da        Isoelectric Point: 7.5062

>T143246 WP_009485244.1 NZ_CP045988:c2639483-2638161 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPICEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T143246 NZ_CP060979:195531-195638 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT143246 WP_002904790.1 NZ_CP045988:c2639749-2639483 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT143246 NZ_CP060979:c195483-195417 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A486MZ73

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References