Detailed information of TA system TA143235

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yjhX-yjhQ/YjhX-GNAT
• Location: 4410911..4411725
• Replicon: chromosome
• Accession: NZ_CP045977
• Organism: Escherichia coli strain INSC1002

Toxin (Protein)

• Gene name: yjhX
• Uniprot ID: S1PA82
• Locus tag: GI529_RS21235
• Protein ID: WP_001054376.1
• Coordinates: 4411468..4411725 (-)
• Length: 86 a.a.

Antitoxin (Protein)

• Gene name: yjhQ
• Uniprot ID: U9Z4B8
• Locus tag: GI529_RS21230
• Protein ID: WP_001309181.1
• Coordinates: 4410911..4411456 (-)
• Length: 182 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GI529_RS21205	4406602..4407915	-	1314	WP_000460843.1	galactitol-specific PTS transporter subunit IIC	-
GI529_RS21210	4407927..4408205	-	279	WP_000722973.1	PTS sugar transporter subunit IIB	-
GI529_RS21215	4408202..4409323	-	1122	WP_000010829.1	M42 family metallopeptidase	-
GI529_RS21920	4409568..4409684	-	117	Protein_4121	VOC family protein	-
GI529_RS21220	4409722..4409940	-	219	Protein_4122	hypothetical protein	-
GI529_RS21225	4410109..4410855	-	747	WP_000354251.1	class I SAM-dependent methyltransferase	-
GI529_RS21230	4410911..4411456	-	546	WP_001309181.1	N-acetyltransferase	Antitoxin
GI529_RS21235	4411468..4411725	-	258	WP_001054376.1	hypothetical protein	Toxin
GI529_RS21240	4412102..4412347	-	246	Protein_4126	GNAT family N-acetyltransferase	-
GI529_RS21245	4412463..4413703	+	1241	Protein_4127	helicase YjhR	-
GI529_RS21250	4414286..4415266	-	981	WP_000991438.1	9-O-acetyl-N-acetylneuraminic acid deacetylase	-
GI529_RS21255	4415331..4416437	-	1107	WP_001309184.1	N-acetylneuraminate epimerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	fimB / fimE	4369322..4421081	51759

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Putative toxin of bacterial toxin-antitoxin pair (1-85)

Antitoxin

• GNAT domain (18-121)

Sequences

Toxin

Protein

• Length: 86 a.a.
• Molecular weight: 9734.29 Da
• Isoelectric Point: 11.0090

>T143235 WP_001054376.1 NZ_CP045977:c4411725-4411468 [Escherichia coli]
MNLSRQEQHTLHVLAKGRRIAHVRDSSGRVTSVECYSREGLLLTDCTLAVFKKLKTKKLIKSVNGQPYRINTTELNKVRA
QLDNR

Nucleotide

• Length: 258 bp

>T143235 NZ_CP060974:3516601-3516819 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 182 a.a.
• Molecular weight: 19956.90 Da
• Isoelectric Point: 6.3277

>AT143235 WP_001309181.1 NZ_CP045977:c4411456-4410911 [Escherichia coli]
MTVHHFTFHITDKSDASDIREVETRAFGFSKEADLVASLLEDESARPALSLLARYEGKAVGHILFTRATFKGEMDSPLMH
ILAPLAVIPEYQGMGVGGRLIRTGIEHLRLMGCQTVFVLGHATYYPRHGFEPCAGDKGYPAPYPIPEEHKACWMMQSLTA
QPMTLTGHIRCADPDETGALT

Nucleotide

• Length: 546 bp

>AT143235 NZ_CP060974:3516201-3516575 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: S1PA82

Antitoxin

• AlphaFold DB: U9Z4B8