Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 47909..48468 Replicon plasmid pAUSMDU00002545_01
Accession NZ_CP045976
Organism Escherichia coli strain AUSMDU00002545

Toxin (Protein)


Gene name relE Uniprot ID O82928
Locus tag GJD97_RS27725 Protein ID WP_000421248.1
Coordinates 47909..48184 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID C1J8K5
Locus tag GJD97_RS27730 Protein ID WP_001178089.1
Coordinates 48184..48468 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJD97_RS27690 43262..43651 - 390 WP_001172748.1 cytochrome b562 family protein -
GJD97_RS27695 43695..45905 - 2211 WP_000592771.1 catalase/peroxidase KatP -
GJD97_RS27700 46082..46267 + 186 WP_001302179.1 hypothetical protein -
GJD97_RS27705 46267..46683 + 417 Protein_41 IS91 family transposase -
GJD97_RS27710 46683..47249 + 567 Protein_42 IS3-like element IS600 family transposase -
GJD97_RS27715 47293..47508 + 216 Protein_43 transposase -
GJD97_RS27720 47608..47814 - 207 WP_001105064.1 hypothetical protein -
GJD97_RS27725 47909..48184 - 276 WP_000421248.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GJD97_RS27730 48184..48468 - 285 WP_001178089.1 ribbon-helix-helix domain-containing protein Antitoxin
GJD97_RS27735 49382..50239 - 858 WP_000130945.1 incFII family plasmid replication initiator RepA -
GJD97_RS27740 50232..50306 - 75 WP_001370046.1 RepA leader peptide Tap -
GJD97_RS27745 50542..50796 - 255 WP_000083831.1 replication regulatory protein RepA -
GJD97_RS27750 51036..51374 - 339 WP_000766796.1 hypothetical protein -
GJD97_RS27755 51412..51621 - 210 WP_001302200.1 hemolysin expression modulator Hha -
GJD97_RS27760 51667..52128 - 462 WP_001233853.1 thermonuclease family protein -
GJD97_RS27765 52373..52585 - 213 WP_001302189.1 ANR family transcriptional regulator -
GJD97_RS27770 52718..53278 - 561 WP_000840472.1 fertility inhibition protein FinO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - hlyD / hlyB / hlyA / hlyC / exeG / exeE / stcE / espP / espP / espP / toxB 1..94640 94640


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10735.42 Da        Isoelectric Point: 9.9324

>T143209 WP_000421248.1 NZ_CP045976:c48184-47909 [Escherichia coli]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T143209 NZ_CP060969:3555165-3555383 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10474.89 Da        Isoelectric Point: 5.6558

>AT143209 WP_001178089.1 NZ_CP045976:c48468-48184 [Escherichia coli]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Download         Length: 285 bp

>AT143209 NZ_CP060969:3554765-3555139 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2Z6FAZ0

Antitoxin

Source ID Structure
AlphaFold DB A0A731IHA8

References