Detailed information of TA system TA143204

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 4697239..4697789
• Replicon: chromosome
• Accession: NZ_CP045975
• Organism: Escherichia coli strain AUSMDU00002545

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: D3H432
• Locus tag: GJD97_RS23590
• Protein ID: WP_001160975.1
• Coordinates: 4697239..4697553 (-)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: GJD97_RS23595
• Protein ID: WP_000125566.1
• Coordinates: 4697556..4697789 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD97_RS23565	4692914..4694200	+	1287	WP_000800457.1	peptidylprolyl isomerase SurA	-
GJD97_RS23570	4694200..4695189	+	990	WP_000241259.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
GJD97_RS23575	4695186..4696007	+	822	WP_001065380.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
GJD97_RS23580	4696010..4696387	+	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
GJD97_RS23585	4696394..4697242	+	849	WP_000257187.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
GJD97_RS23590	4697239..4697553	-	315	WP_001160975.1	CcdB family protein	Toxin
GJD97_RS23595	4697556..4697789	-	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GJD97_RS23600	4697875..4698354	-	480	WP_000624372.1	type 3 dihydrofolate reductase	-
GJD97_RS23605	4698546..4700408	-	1863	WP_072616958.1	glutathione-regulated potassium-efflux system protein KefC	-
GJD97_RS23610	4700401..4700931	-	531	WP_000600700.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
GJD97_RS23615	4701039..4702370	-	1332	WP_001183177.1	MFS transporter	-
GJD97_RS23620	4702428..4702715	-	288	WP_000203741.1	ferredoxin-like protein FixX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-103)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 11711.76 Da
• Isoelectric Point: 9.8517

>T143204 WP_001160975.1 NZ_CP045975:c4697553-4697239 [Escherichia coli]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Nucleotide

• Length: 315 bp

>T143204 NZ_CP060969:2055136-2055239 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT143204 WP_000125566.1 NZ_CP045975:c4697789-4697556 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT143204 NZ_CP060969:c2055277-2055006 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9