Detailed information of TA system TA143197

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2783323..2783961
• Replicon: chromosome
• Accession: NZ_CP045975
• Organism: Escherichia coli strain AUSMDU00002545

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: GJD97_RS14090
• Protein ID: WP_000813794.1
• Coordinates: 2783785..2783961 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: GJD97_RS14085
• Protein ID: WP_001270286.1
• Coordinates: 2783323..2783739 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD97_RS14065	2778475..2779416	-	942	WP_001251315.1	ABC transporter permease	-
GJD97_RS14070	2779417..2780430	-	1014	WP_000220402.1	ABC transporter ATP-binding protein	-
GJD97_RS14075	2780448..2781593	-	1146	WP_000047432.1	ABC transporter substrate-binding protein	-
GJD97_RS14080	2781838..2783244	-	1407	WP_000760654.1	PLP-dependent aminotransferase family protein	-
GJD97_RS14085	2783323..2783739	-	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
GJD97_RS14090	2783785..2783961	-	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
GJD97_RS14095	2784183..2784413	+	231	WP_000494244.1	YncJ family protein	-
GJD97_RS14100	2784505..2786466	-	1962	WP_001303492.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
GJD97_RS14105	2786539..2787075	-	537	WP_000429145.1	DNA-binding transcriptional regulator SutR	-
GJD97_RS14110	2787128..2788339	+	1212	WP_071525382.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T143197 WP_000813794.1 NZ_CP045975:c2783961-2783785 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T143197 NZ_CP060969:751125-751508 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCACGCCCGTCTCCGGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGTATTGATATTCTCCGGGCTCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCAGGAGTCGAAACGATGCAACTGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT143197 WP_001270286.1 NZ_CP045975:c2783739-2783323 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT143197 NZ_CP060969:750667-751035 [Escherichia coli]
GTGTCAGACACATTCTCCGGGACAACGCATCCCGATGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGTACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTACAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGATGCGGATGCGAACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCAGT
GAACTGAATCCCCACCGTCAGAATACCGTCACGCTGTATGTTAAAGGGCTGACCTGCCATGCGGACACCCTCGGCAGCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Toxin

• PDB: 6HPB