Detailed information of TA system TA143178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 2345570..2347291
• Replicon: chromosome
• Accession: NZ_CP045974
• Organism: Legionella pneumophila strain AUSMDU00010536 isolate SBT211

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: -
• Locus tag: GJD98_RS10595
• Protein ID: WP_038839099.1
• Coordinates: 2345570..2346502 (-)
• Length: 311 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: A0A128I8L5
• Locus tag: GJD98_RS10600
• Protein ID: WP_015444253.1
• Coordinates: 2346506..2347291 (-)
• Length: 262 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD98_RS10570	2341129..2341482	-	354	WP_015444259.1	conjugal transfer transcriptional regulator TraJ	-
GJD98_RS10575	2341876..2342220	+	345	WP_015444258.1	TraK family protein	-
GJD98_RS10580	2342220..2342945	+	726	WP_015444257.1	IncP-type DNA transfer protein TraL	-
GJD98_RS10585	2342945..2343382	+	438	WP_015444256.1	conjugal transfer protein TraM	-
GJD98_RS10590	2343497..2345437	+	1941	WP_038839098.1	DUF2779 domain-containing protein	-
GJD98_RS10595	2345570..2346502	-	933	WP_038839099.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
GJD98_RS10600	2346506..2347291	-	786	WP_015444253.1	type IV toxin-antitoxin system AbiEi family antitoxin	Antitoxin
GJD98_RS10605	2347776..2348780	-	1005	WP_010947770.1	site-specific integrase	-
GJD98_RS10610	2348693..2348995	-	303	WP_010947771.1	helix-turn-helix domain-containing protein	-
GJD98_RS10615	2349405..2350028	+	624	WP_010947772.1	helix-turn-helix domain-containing protein	-
GJD98_RS10620	2350025..2350231	-	207	WP_021436844.1	hypothetical protein	-
GJD98_RS10625	2350392..2351699	+	1308	WP_010947773.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2270978..2411920	140942

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (13-264)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (97-254)

Sequences

Toxin

Protein

• Length: 311 a.a.
• Molecular weight: 35330.14 Da
• Isoelectric Point: 6.8643

>T143178 WP_038839099.1 NZ_CP045974:c2346502-2345570 [Legionella pneumophila]
MNKQSPYYKQVLLLIRLIPLVEKETCFALKGGTAINLFVRDFPRLSVDIDLAYLPLEPREIALANVRNALAGIAANINKI
PDLNAVLQDNKPDEMRIIVEDHSSLNRGTQIKIEVSPVARGTLLPPVELDVVASVENEFGFASMKVVSLPDLYGGKLCAA
LDRQHPRDLFDVKVLLENQKIDRELFNGFLTYLLSHKRPISEIMNPRWKDITQVFEREFKGMTAEPVTLEELTAIPGKMI
QGLKDNFTQQDYDFLVSFKRGEPEWSLACSDQIQYLPAVQWKLLNIRKMPKQKLMDSVSLLQRTMGHWLN

Nucleotide

• Length: 933 bp

>T143178 NZ_CP060963:c1797922-1797548 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACGCTGAATGACACTCCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCCGGCATTTCACTGTGCGATGCAGTGAACTTTCTTGTTGAAAAATACGCACTGGTACGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 262 a.a.
• Molecular weight: 29773.22 Da
• Isoelectric Point: 9.4457

>AT143178 WP_015444253.1 NZ_CP045974:c2347291-2346506 [Legionella pneumophila]
MSSKINWLLNNIASGEILLQSWLSSHGITPQLARKYCQSQWLVKLGVGVYYRAGRKPRWQNAVYCLQNQSRYMIHLAGLT
SLAQQGKSHYLQFAPDSIWINVQAKTILPKWFKNFPELVISDELIPNWEIIKTSKLNAQDENDLTTIEVDGIKLKASNQE
LAAFEVLEAVPSTLSFEHAAELFQGLVNLSPRKVEHILKRSGSIQTNRLFLFLGHFYKHPWAERVNESLINLGAGKRQIV
PDGKLEKQYQITVPEQFISSD

Nucleotide

• Length: 786 bp

>AT143178 NZ_CP060963:c1798379-1798011 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACTCATCCCGATGACAACAACAGCCACCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCCTGTCTGGTGCAGGAGGGTAACCGGTTGCATTATCTTGCCGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA

Structures

Antitoxin

• AlphaFold DB: A0A128I8L5