Detailed information of TA system TA143177

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF-MazE
• Location: 37478..38055
• Replicon: plasmid pAUSMDU00000224_01
• Accession: NZ_CP045973
• Organism: Listeria monocytogenes strain AUSMDU00000224

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: H1G8D6
• Locus tag: GJD99_RS14885
• Protein ID: WP_003728465.1
• Coordinates: 37478..37819 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: H1G8D5
• Locus tag: GJD99_RS14890
• Protein ID: WP_003728464.1
• Coordinates: 37813..38055 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJD99_RS14870	33957..34511	-	555	WP_003728468.1	recombinase family protein	-
GJD99_RS14875	34791..35150	+	360	WP_003728467.1	Cd(II)-sensing metalloregulatory transcriptional repressor CadC	-
GJD99_RS14880	35150..37285	+	2136	WP_003728466.1	cadmium-translocating P-type ATPase	-
GJD99_RS14885	37478..37819	-	342	WP_003728465.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GJD99_RS14890	37813..38055	-	243	WP_003728464.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GJD99_RS14895	38116..38367	-	252	WP_003728463.1	hypothetical protein	-
GJD99_RS14900	38498..38824	+	327	WP_003728462.1	hypothetical protein	-
GJD99_RS14905	38824..39486	+	663	WP_003731676.1	DUF2786 domain-containing protein	-
GJD99_RS14910	39505..39882	+	378	WP_003769263.1	DUF3850 domain-containing protein	-
GJD99_RS14915	40041..40529	+	489	WP_003728520.1	hypothetical protein	-
GJD99_RS14920	40651..40899	+	249	WP_003728519.1	hypothetical protein	-
GJD99_RS14925	40900..41325	+	426	WP_003728518.1	hypothetical protein	-
GJD99_RS14930	41329..42051	+	723	WP_003728517.1	CRISPR-associated protein Cas5	-
GJD99_RS14935	42067..42783	+	717	WP_015055880.1	AP2 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..57553	57553

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-84)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 16796.15 Da
• Isoelectric Point: 5.5500

>T143177 WP_003728465.1 NZ_CP045973:c37819-37478 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF

Nucleotide

• Length: 342 bp

>T143177 NZ_CP060963:1786026-1786280 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 11939.32 Da
• Isoelectric Point: 5.5500

>AT143177 WP_003728464.1 NZ_CP045973:c38055-37813 [Listeria monocytogenes]
FFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFFF
F

Nucleotide

• Length: 243 bp

>AT143177 NZ_CP060963:1785778-1786029 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: H1G8D6

Antitoxin

• AlphaFold DB: H1G8D5