Detailed information of TA system TA143172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0298-Rv0299/-
• Location: 4646535..4647061
• Replicon: chromosome
• Accession: NZ_CP045963
• Organism: Mycobacterium intracellulare subsp. chimaera strain AUSMDU00007395

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: S4ZDZ6
• Locus tag: GJE02_RS21470
• Protein ID: WP_008259048.1
• Coordinates: 4646759..4647061 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: Rv0298
• Uniprot ID: S4ZFD0
• Locus tag: GJE02_RS21465
• Protein ID: WP_008259047.1
• Coordinates: 4646535..4646762 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE02_RS21440	4641606..4642532	-	927	WP_042912749.1	MinD/ParA family protein	-
GJE02_RS21445	4642777..4643808	-	1032	WP_042912751.1	6-phosphofructokinase	-
GJE02_RS21450	4643852..4645345	-	1494	WP_042912754.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatA	-
GJE02_RS21455	4645342..4645641	-	300	WP_009952003.1	Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase subunit GatC	-
GJE02_RS21460	4645741..4646397	+	657	WP_087139897.1	amino acid-binding protein	-
GJE02_RS21465	4646535..4646762	+	228	WP_008259047.1	ribbon-helix-helix protein, CopG family	Antitoxin
GJE02_RS21470	4646759..4647061	+	303	WP_008259048.1	hypothetical protein	Toxin
GJE02_RS21475	4647177..4647485	-	309	WP_008259049.1	hypothetical protein	-
GJE02_RS21480	4647544..4650465	-	2922	WP_014712101.1	MMPL family transporter	-
GJE02_RS21485	4650462..4650866	-	405	WP_008259052.1	hypothetical protein	-
GJE02_RS21490	4651113..4651631	+	519	WP_042912757.1	pyridoxamine 5'-phosphate oxidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (6-41)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10473.31 Da
• Isoelectric Point: 4.5636

>T143172 WP_008259048.1 NZ_CP045963:4646759-4647061 [Mycobacterium intracellulare subsp. chimaera]
MIAPGDIAPRRDTRRDLYVAVLSNAIHLAANTGRVIVCPFIPGKIPDDTMAMVVPLTQPEGTLLPELVQWLPTSALGEPI
GAVSAAALSEAMSIVTALIT

Nucleotide

• Length: 303 bp

>T143172 NZ_CP060963:648091-648405 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8173.14 Da
• Isoelectric Point: 6.4608

>AT143172 WP_008259047.1 NZ_CP045963:4646535-4646762 [Mycobacterium intracellulare subsp. chimaera]
MAKEKISVTVDAAVLADADSDAKAAGLNRSEMIERALRNEHLRRALHTYTTRTAPALNIDSYAGQVYEANRAADL

Nucleotide

• Length: 228 bp

>AT143172 NZ_CP060963:648402-648818 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• AlphaFold DB: S4ZDZ6

Antitoxin

• AlphaFold DB: S4ZFD0