Detailed information of TA system TA143166

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1358285..1358938
• Replicon: chromosome
• Accession: NZ_CP045963
• Organism: Mycobacterium intracellulare subsp. chimaera strain AUSMDU00007395

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: GJE02_RS06765
• Protein ID: WP_008253911.1
• Coordinates: 1358477..1358938 (+)
• Length: 154 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: X8A187
• Locus tag: GJE02_RS06760
• Protein ID: WP_009957321.1
• Coordinates: 1358285..1358473 (+)
• Length: 63 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE02_RS06750	1354258..1355835	+	1578	WP_042911067.1	serine hydrolase	-
GJE02_RS06755	1355832..1358219	+	2388	WP_042910814.1	cation-translocating P-type ATPase	-
GJE02_RS06760	1358285..1358473	+	189	WP_009957321.1	antitoxin	Antitoxin
GJE02_RS06765	1358477..1358938	+	462	WP_008253911.1	SRPBCC family protein	Toxin
GJE02_RS06770	1359025..1359795	+	771	WP_042910815.1	VOC family protein	-
GJE02_RS06775	1359869..1360303	+	435	WP_008253914.1	hypothetical protein	-
GJE02_RS06780	1360308..1360796	-	489	WP_036454690.1	glyoxalase/bleomycin resistance/dioxygenase family protein	-
GJE02_RS06785	1360796..1362310	-	1515	WP_042910816.1	carotenoid oxygenase family protein	-
GJE02_RS06790	1362313..1363533	-	1221	WP_042910817.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-52)

Sequences

Toxin

Protein

• Length: 154 a.a.
• Molecular weight: 16639.20 Da
• Isoelectric Point: 8.5617

>T143166 WP_008253911.1 NZ_CP045963:1358477-1358938 [Mycobacterium intracellulare subsp. chimaera]
MAKLSGSIDVPLPPEVAWQHASDLSRYKDWLTIHRVWRSALPDEIEKGSVVESIVEVKGMPNRIKWTVVRYKPPEGMTLN
GDGVGGVKVKLMAKIQPKGDGSLVSFDVHLGGPALFGPIGMIVAAALRGDIDQSLENFVTVFTRPDPSSNGHR

Nucleotide

• Length: 462 bp

>T143166 NZ_CP060953:4169886-4170188 [Escherichia coli]
ATGGCTCAAAAGAAAAATATAAGGAGTTTTCGTGATGCCTGGTTGGCTGATTTTTTTGTCCATTCAACGCCACATCGAAA
GATCCCTGCTGAAATCCATACCACGTTATCACGAAAGTTGGACATCATTAATGCTGCCACTTCTCATCGGGATTTACGCT
CCCCTCCGGGGAACCGATACGAAGAGTTATCCGGGAAATTACAGGAATACTCCTCCATTAGAGTGAATAAACAGTACCGA
CTGATTTTTAAATGGGTTAATGGCAAGGCCGAAGAATTGTTTTTAGACCCACACAACTACTAA

Antitoxin

Protein

• Length: 63 a.a.
• Molecular weight: 6698.30 Da
• Isoelectric Point: 4.4702

>AT143166 WP_009957321.1 NZ_CP045963:1358285-1358473 [Mycobacterium intracellulare subsp. chimaera]
MGFLDKAKDLLSQNADKVETAIDKAGEFVDEKTQGKYSDTIHQVQEQAKKAAGAADTGDQQN

Nucleotide

• Length: 189 bp

>AT143166 NZ_CP060953:4170224-4170565 [Escherichia coli]
ATGAAACAGGCAACAAGAAAACCGACGACACCCGGAGATATTCTTCTCTATGAATATCTGGAACCGCTCGATTTGAAAAT
CAATGAGTTAGCAGAGTTGCTGCATGTTCATCGTAATAGCGTCAGTGCACTGATCAATAACAATCGTAAACTCACCACTG
AGATGGCATTCCGTCTGGCGAAAGTTTTTGATACCACAGTCGATTTTTGGCTAAACCTCCAGGCGGCAGTTGATCTTTGG
GAAGTTGAAAACAACATGCGTACCCAGGAAGAATTGGGACGGATTGAAACAGTGGCTGAATATTTGGCACGCCGTGAAGA
GCGTGCAAAAAAGGTCGCGTAG

Structures

Antitoxin

• AlphaFold DB: X8A187