Detailed information of TA system TA143165

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PHD(antitoxin)
• Location: 1137090..1137773
• Replicon: chromosome
• Accession: NZ_CP045963
• Organism: Mycobacterium intracellulare subsp. chimaera strain AUSMDU00007395

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: D5PEA4
• Locus tag: GJE02_RS05615
• Protein ID: WP_007168487.1
• Coordinates: 1137090..1137497 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1X1XBB9
• Locus tag: GJE02_RS05620
• Protein ID: WP_033711258.1
• Coordinates: 1137501..1137773 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE02_RS29925	1132371..1132586	+	216	WP_158514285.1	hypothetical protein	-
GJE02_RS29930	1132525..1132770	+	246	WP_080711236.1	hypothetical protein	-
GJE02_RS05585	1132746..1133057	+	312	WP_075362207.1	transposase	-
GJE02_RS05590	1133073..1134326	+	1254	WP_101930934.1	IS30 family transposase	-
GJE02_RS05595	1134443..1134613	-	171	Protein_1102	carnitinyl-CoA dehydratase	-
GJE02_RS29935	1134771..1134914	+	144	WP_155250266.1	hypothetical protein	-
GJE02_RS05605	1136362..1136667	-	306	WP_007168485.1	hypothetical protein	-
GJE02_RS05610	1136728..1137069	-	342	WP_007168486.1	hypothetical protein	-
GJE02_RS05615	1137090..1137497	-	408	WP_007168487.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJE02_RS05620	1137501..1137773	-	273	WP_033711258.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
GJE02_RS05625	1138269..1139504	-	1236	WP_042909886.1	IS256 family transposase	-
GJE02_RS05630	1140146..1140331	+	186	WP_132160383.1	hypothetical protein	-
GJE02_RS05635	1140405..1140821	-	417	WP_007168492.1	type II toxin-antitoxin system VapC family toxin	-
GJE02_RS05640	1140818..1141069	-	252	WP_007168493.1	ribbon-helix-helix protein, CopG family	-
GJE02_RS05650	1141871..1142266	+	396	WP_007168495.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1116448..1159082	42634
	-	flank	IS/Tn	-	-	1138269..1139504	1235

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-121)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 14464.55 Da
• Isoelectric Point: 4.6697

>T143165 WP_007168487.1 NZ_CP045963:c1137497-1137090 [Mycobacterium intracellulare subsp. chimaera]
VIYLDTSALVKLIRIEVESDALADWLDERTETRWITSALAEVELPRAIRAVAPNGLSAVPSVLARLDRFEIDQVIRATAA
AYPDPALRSLDAIHLATAQIAASTAPLTAMVAYDSRLSDAARALGITIVTPGRNS

Nucleotide

• Length: 408 bp

>T143165 NZ_CP060953:c4142101-4141883 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9764.35 Da
• Isoelectric Point: 8.7382

>AT143165 WP_033711258.1 NZ_CP045963:c1137773-1137501 [Mycobacterium intracellulare subsp. chimaera]
MSEVPLRMLNQETAKVLARVKRGEEITLTERGEVIARIIPATAGPLDALITAGRVQPATLAGPAPRPTVPMHDNLDAGAL
LQRLRAEERY

Nucleotide

• Length: 273 bp

>AT143165 NZ_CP060953:c4142501-4142127 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: A0A1X1XAJ9

Antitoxin

• AlphaFold DB: A0A1X1XBB9