Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/Txe-YefM
Location 3773271..3773800 Replicon chromosome
Accession NZ_CP045962
Organism Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)


Gene name relE Uniprot ID G0TI95
Locus tag GJE03_RS17685 Protein ID WP_003417760.1
Coordinates 3773543..3773800 (+) Length 86 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G0TI94
Locus tag GJE03_RS17680 Protein ID WP_003417757.1
Coordinates 3773271..3773546 (+) Length 92 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE03_RS20900 3770069..3771506 - 1438 Protein_3451 FAD-binding oxidoreductase -
GJE03_RS17665 3771609..3771998 + 390 WP_003417745.1 DUF732 domain-containing protein -
GJE03_RS17670 3772012..3772305 - 294 WP_003417749.1 DUF3017 domain-containing protein -
GJE03_RS17675 3772302..3773147 - 846 WP_003905054.1 bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase -
GJE03_RS17680 3773271..3773546 + 276 WP_003417757.1 type II toxin-antitoxin system antitoxin RelJ Antitoxin
GJE03_RS17685 3773543..3773800 + 258 WP_003417760.1 Txe/YoeB family addiction module toxin Toxin
GJE03_RS17690 3773842..3775032 + 1191 WP_003900033.1 NADH:flavin oxidoreductase -
GJE03_RS17695 3775149..3775517 + 369 WP_003417765.1 FHA domain-containing protein -
GJE03_RS17700 3775514..3776065 - 552 WP_003417767.1 pentapeptide repeat protein MfpA -
GJE03_RS17705 3776072..3776653 - 582 WP_031662192.1 ATP/GTP-binding protein -
GJE03_RS17710 3776634..3777002 - 369 WP_003417772.1 DUF742 domain-containing protein -
GJE03_RS17715 3776980..3777372 - 393 WP_003417776.1 roadblock/LC7 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(6-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-72)


Sequences

Toxin        


Download         Length: 86 a.a.        Molecular weight: 10070.30 Da        Isoelectric Point: 6.4693

>T143159 WP_003417760.1 NZ_CP045962:3773543-3773800 [Mycobacterium tuberculosis]
VRSVNFDPDAWEDFLFWLAADRKTARRITRLIGEIQRDPFSGIGKPEPLQGELSGYWSRRIDDEHRLVYRAGDDEVTMLK
ARYHY

Download         Length: 258 bp

>T143159 NZ_CP060953:c3255467-3255093 [Escherichia coli]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGAGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTACTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTCGCCGATGAACGTGTGATTGAGCAGCATATTG
AAGCTGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGAAACGATGA

Antitoxin


Download         Length: 92 a.a.        Molecular weight: 10194.35 Da        Isoelectric Point: 4.6652

>AT143159 WP_003417757.1 NZ_CP045962:3773271-3773546 [Mycobacterium tuberculosis]
MSISASEARQRLFPLIEQVNTDHQPVRITSRAGDAVLMSADDYDAWQETVYLLRSPENARRLMEAVARDKAGHSAFTKSV
DELREMAGGEE

Download         Length: 276 bp

>AT143159 NZ_CP060953:c3255925-3255557 [Escherichia coli]
GTGTCAGACAAACTCTCCGGGATAACTCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTTCCCTGCACAGTGAG
GCCCTGTTTTGGTGCCCGTCTGGTGCAGGAGGGTAACCGCCTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGCCGCT
TCAGCGACGCAGATGCATACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCGAAAGGTCTGACCTGCAAAGCTGACACCCTCGGTAGCGG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3OEI

Antitoxin

Source ID Structure
PDB 3CTO
PDB 3OEI
PDB 3D55
AlphaFold DB G0TI94

References