Detailed information of TA system TA143157

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3178933..3179620
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: GJE03_RS15055
• Protein ID: WP_003414624.1
• Coordinates: 3179177..3179620 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: GJE03_RS15050
• Protein ID: WP_003414620.1
• Coordinates: 3178933..3179190 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS15030	3174253..3175107	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
GJE03_RS15035	3175163..3176326	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
GJE03_RS15040	3176343..3177557	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
GJE03_RS15045	3177565..3178806	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
GJE03_RS15050	3178933..3179190	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
GJE03_RS15055	3179177..3179620	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJE03_RS15060	3179700..3180362	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
GJE03_RS15065	3180458..3180649	+	192	WP_003414632.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
GJE03_RS15070	3180981..3182729	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
GJE03_RS15075	3182825..3183406	+	582	WP_003414644.1	fasciclin domain-containing protein	-
GJE03_RS15080	3183506..3183772	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T143157 WP_003414624.1 NZ_CP045962:3179177-3179620 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T143157 NZ_CP060953:2945460-2945771 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGAACGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTCTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT143157 WP_003414620.1 NZ_CP045962:3178933-3179190 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT143157 NZ_CP060953:2945772-2946062 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
ACGTACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0