Detailed information of TA system TA143156

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 3173332..3173880
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G0TFG5
• Locus tag: GJE03_RS15025
• Protein ID: WP_003414602.1
• Coordinates: 3173617..3173880 (+)
• Length: 88 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: O33347
• Locus tag: GJE03_RS15020
• Protein ID: WP_003414599.1
• Coordinates: 3173332..3173613 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS14995	3168955..3169812	-	858	WP_003899513.1	type I methionyl aminopeptidase	-
GJE03_RS15000	3169854..3170438	-	585	WP_003414585.1	DUF1707 domain-containing protein	-
GJE03_RS15005	3170542..3170790	+	249	WP_003913411.1	antitoxin VapB23	-
GJE03_RS15010	3170787..3171167	+	381	WP_154079986.1	type II toxin-antitoxin system VapC family toxin	-
GJE03_RS15015	3171249..3173060	-	1812	WP_003414596.1	penicillin-binding protein	-
GJE03_RS15020	3173332..3173613	+	282	WP_003414599.1	type II toxin-antitoxin system antitoxin RelF	Antitoxin
GJE03_RS15025	3173617..3173880	+	264	WP_003414602.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GJE03_RS15030	3174253..3175107	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
GJE03_RS15035	3175163..3176326	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
GJE03_RS15040	3176343..3177557	-	1215	WP_003414610.1	zinc metalloprotease Rip	-
GJE03_RS15045	3177565..3178806	-	1242	WP_003414613.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-85)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-72)

Sequences

Toxin

Protein

• Length: 88 a.a.
• Molecular weight: 10204.82 Da
• Isoelectric Point: 11.5078

>T143156 WP_003414602.1 NZ_CP045962:3173617-3173880 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Nucleotide

• Length: 264 bp

>T143156 NZ_CP060953:2103078-2103542 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGTTCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10183.40 Da
• Isoelectric Point: 4.3440

>AT143156 WP_003414599.1 NZ_CP045962:3173332-3173613 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Nucleotide

• Length: 282 bp

>AT143156 NZ_CP060953:2102743-2103078 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• PDB: 3G5O

Antitoxin

• AlphaFold DB: A0A7U4BWP8