143155

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN_Sll0205-like-Phd
Location 3132453..3133057 Replicon chromosome
Accession NZ_CP045962
Organism Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)


Gene name vapC Uniprot ID P71623
Locus tag GJE03_RS14820 Protein ID WP_003414492.1
Coordinates 3132453..3132845 (-) Length 131 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A829CBY8
Locus tag GJE03_RS14825 Protein ID WP_003414495.1
Coordinates 3132842..3133057 (-) Length 72 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE03_RS14790 3127603..3128391 - 789 WP_003917092.1 CRISPR-associated endoribonuclease Cas6 -
GJE03_RS14795 3128725..3129270 - 546 WP_003904931.1 DUF1802 family protein -
GJE03_RS14800 3129542..3130426 - 885 WP_003414409.1 nucleotidyl transferase AbiEii/AbiGii toxin family protein -
GJE03_RS14805 3130429..3131316 - 888 WP_003414414.1 type IV toxin-antitoxin system AbiEi family antitoxin -
GJE03_RS14810 3131621..3132166 - 546 WP_003904931.1 DUF1802 family protein -
GJE03_RS14815 3132163..3132432 - 270 WP_003414489.1 DUF2277 family protein -
GJE03_RS14820 3132453..3132845 - 393 WP_003414492.1 type II toxin-antitoxin system VapC family toxin Toxin
GJE03_RS14825 3132842..3133057 - 216 WP_003414495.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GJE03_RS14830 3133104..3133853 + 750 WP_003414497.1 enoyl-CoA hydratase -
GJE03_RS14835 3133932..3135014 - 1083 WP_003414499.1 ABC transporter ATP-binding protein -
GJE03_RS14840 3135007..3136317 - 1311 WP_003414501.1 ABC transporter substrate-binding protein -
GJE03_RS14845 3136320..3137147 - 828 WP_003414504.1 carbohydrate ABC transporter permease -
GJE03_RS14850 3137144..3138055 - 912 WP_003414505.1 sugar ABC transporter permease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

VapC-like Sll0205 protein, PIN domain

(5-130)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(2-46)


Sequences

Toxin        


Download         Length: 131 a.a.        Molecular weight: 14610.74 Da        Isoelectric Point: 6.7594

>T143155 WP_003414492.1 NZ_CP045962:c3132845-3132453 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Download         Length: 393 bp

>T143155 NZ_CP060953:c2060196-2059882 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCATCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTTGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCTGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin


Download         Length: 72 a.a.        Molecular weight: 7511.55 Da        Isoelectric Point: 4.9732

>AT143155 WP_003414495.1 NZ_CP045962:c3133057-3132842 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Download         Length: 216 bp

>AT143155 NZ_CP060953:c2059885-2059469 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTGCTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGCGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTGCCGGAAATTGGCAGTAAATCTATGGT
GTCACGCGTTTTGAGCGGGAAGAGGAAATTAACGCTGGAACACGCTAAAAAATTGGCAACGCGATTCGGCATTTCTCCCG
CCTTGTTTATTGATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BWM8

Antitoxin

Source ID Structure
AlphaFold DB A0A829CBY8

References