Detailed information of TA system TA143153

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 3107636..3108206
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: P71650
• Locus tag: GJE03_RS14680
• Protein ID: WP_003414166.1
• Coordinates: 3107636..3107992 (-)
• Length: 119 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P0CL61
• Locus tag: GJE03_RS14685
• Protein ID: WP_003901465.1
• Coordinates: 3107976..3108206 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS14660	3103088..3104776	-	1689	WP_003414155.1	alpha/beta hydrolase family protein	-
GJE03_RS14665	3104780..3105106	-	327	WP_003414157.1	hypothetical protein	-
GJE03_RS14670	3105279..3105866	+	588	WP_003914429.1	DUF3558 family protein	-
GJE03_RS14675	3105885..3107534	+	1650	WP_003899485.1	CocE/NonD family hydrolase	-
GJE03_RS14680	3107636..3107992	-	357	WP_003414166.1	type II toxin-antitoxin system toxin endoribonuclease MazF9	Toxin
GJE03_RS14685	3107976..3108206	-	231	WP_003901465.1	antitoxin MazE	Antitoxin
GJE03_RS14690	3108249..3109292	-	1044	WP_003414172.1	DUF2293 domain-containing protein	-
GJE03_RS14695	3109483..3109758	+	276	WP_003911993.1	DUF1778 domain-containing protein	-
GJE03_RS14700	3109934..3110188	-	255	WP_003917684.1	hypothetical protein	-
GJE03_RS14705	3110336..3110740	+	405	WP_003414181.1	hypothetical protein	-
GJE03_RS14710	3110737..3110928	+	192	WP_003414184.1	hypothetical protein	-
GJE03_RS14715	3110992..3112281	+	1290	Protein_2882	transposase family protein	-
GJE03_RS14720	3112515..3112772	+	258	WP_003899489.1	hypothetical protein	-
GJE03_RS14725	3112877..3113188	+	312	WP_003414190.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-113)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-29)

Sequences

Toxin

Protein

• Length: 119 a.a.
• Molecular weight: 12858.73 Da
• Isoelectric Point: 9.1962

>T143153 WP_003414166.1 NZ_CP045962:c3107992-3107636 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Nucleotide

• Length: 357 bp

>T143153 NZ_CP060953:c1786861-1786454 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGCATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8231.01 Da
• Isoelectric Point: 4.1809

>AT143153 WP_003901465.1 NZ_CP045962:c3108206-3107976 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Nucleotide

• Length: 231 bp

>AT143153 NZ_CP060953:c1787108-1786842 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATTCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6L2A
• PDB: 5HJZ
• PDB: 6KYT
• PDB: 6L29
• PDB: 7DU5
• PDB: 6KYS

Antitoxin

• AlphaFold DB: A0A7U4FBW3