Detailed information of TA system TA143151

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3067638..3068317
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: GJE03_RS14455
• Protein ID: WP_003414059.1
• Coordinates: 3067638..3068054 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: GJE03_RS14460
• Protein ID: WP_003414061.1
• Coordinates: 3068051..3068317 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS14435	3063690..3064592	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
GJE03_RS14440	3064661..3065413	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
GJE03_RS14445	3065657..3065932	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
GJE03_RS14450	3065929..3067551	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
GJE03_RS14455	3067638..3068054	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
GJE03_RS14460	3068051..3068317	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GJE03_RS14465	3068343..3068738	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
GJE03_RS14470	3068735..3069004	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
GJE03_RS14475	3069014..3070108	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
GJE03_RS14480	3070105..3070524	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
GJE03_RS14485	3070598..3071077	-	480	WP_003414073.1	dihydrofolate reductase	-
GJE03_RS14490	3071148..3071948	-	801	WP_003911953.1	thymidylate synthase	-
GJE03_RS14495	3072104..3072841	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T143151 WP_003414059.1 NZ_CP045962:c3068054-3067638 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T143151 NZ_CP060953:c822380-822129 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT143151 WP_003414061.1 NZ_CP045962:c3068317-3068051 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT143151 NZ_CP060953:c822132-821878 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64