Detailed information of TA system TA143150

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2938454..2939168
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: GJE03_RS13705
• Protein ID: WP_003413460.1
• Coordinates: 2938728..2939168 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ20
• Locus tag: GJE03_RS13700
• Protein ID: WP_003413456.1
• Coordinates: 2938454..2938741 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS13665	2933876..2934121	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GJE03_RS13670	2934118..2934522	+	405	WP_003413432.1	type II toxin-antitoxin system VapC family toxin	-
GJE03_RS13675	2934739..2935359	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
GJE03_RS13680	2935370..2935864	+	495	WP_003413444.1	DUF2617 family protein	-
GJE03_RS13685	2935861..2936292	+	432	WP_003413445.1	DUF4247 domain-containing protein	-
GJE03_RS13690	2936317..2936775	+	459	WP_003413451.1	DUF350 domain-containing protein	-
GJE03_RS13695	2936772..2938343	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
GJE03_RS13700	2938454..2938741	+	288	WP_003413456.1	antitoxin VapB41	Antitoxin
GJE03_RS13705	2938728..2939168	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJE03_RS13710	2939189..2939944	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
GJE03_RS13715	2940077..2940673	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
GJE03_RS13720	2940681..2941526	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
GJE03_RS13725	2941555..2942454	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
GJE03_RS13730	2942582..2943256	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T143150 WP_003413460.1 NZ_CP045962:2938728-2939168 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T143150 NZ_CP060953:c822132-821878 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10453.98 Da
• Isoelectric Point: 7.2797

>AT143150 WP_003413456.1 NZ_CP045962:2938454-2938741 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT143150 NZ_CP060953:c822380-822129 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Structures

Toxin

• AlphaFold DB: G0TQK0

Antitoxin

• AlphaFold DB: A0A7U4BWB8