143149

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2878102..2878739 Replicon chromosome
Accession NZ_CP045962
Organism Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag GJE03_RS13440 Protein ID WP_003413180.1
Coordinates 2878102..2878497 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag GJE03_RS13445 Protein ID WP_003413183.1
Coordinates 2878494..2878739 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE03_RS13400 2874187..2874846 + 660 WP_003900846.1 LppA family lipoprotein -
GJE03_RS13405 2874843..2875502 + 660 WP_003900847.1 hypothetical protein -
GJE03_RS13410 2875499..2876161 + 663 WP_055351404.1 hypothetical protein -
GJE03_RS13415 2876158..2876436 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
GJE03_RS13420 2876529..2876942 + 414 WP_003413164.1 PIN domain nuclease -
GJE03_RS13425 2876981..2877238 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
GJE03_RS13430 2877235..2877612 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
GJE03_RS13435 2877628..2878002 - 375 WP_003413177.1 hypothetical protein -
GJE03_RS13440 2878102..2878497 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
GJE03_RS13445 2878494..2878739 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
GJE03_RS13450 2879150..2879569 - 420 WP_003413190.1 A24 family peptidase -
GJE03_RS13455 2879581..2880390 - 810 WP_003413193.1 shikimate dehydrogenase -
GJE03_RS13460 2880387..2881640 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
GJE03_RS13465 2881633..2882145 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T143149 WP_003413180.1 NZ_CP045962:c2878497-2878102 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T143149 NZ_CP060953:c548709-548607 [Escherichia coli]
GCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT143149 WP_003413183.1 NZ_CP045962:c2878739-2878494 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT143149 NZ_CP060953:548569-548837 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCTCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References