Detailed information of TA system TA143148

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 2876981..2877612
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ28
• Locus tag: GJE03_RS13430
• Protein ID: WP_003413174.1
• Coordinates: 2877235..2877612 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: GJE03_RS13425
• Protein ID: WP_003413167.1
• Coordinates: 2876981..2877238 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS13390	2872239..2872553	+	315	WP_009937839.1	hypothetical protein	-
GJE03_RS13395	2872849..2874060	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
GJE03_RS13400	2874187..2874846	+	660	WP_003900846.1	LppA family lipoprotein	-
GJE03_RS13405	2874843..2875502	+	660	WP_003900847.1	hypothetical protein	-
GJE03_RS13410	2875499..2876161	+	663	WP_055351404.1	hypothetical protein	-
GJE03_RS13415	2876158..2876436	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
GJE03_RS13420	2876529..2876942	+	414	WP_003413164.1	PIN domain nuclease	-
GJE03_RS13425	2876981..2877238	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
GJE03_RS13430	2877235..2877612	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJE03_RS13435	2877628..2878002	-	375	WP_003413177.1	hypothetical protein	-
GJE03_RS13440	2878102..2878497	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
GJE03_RS13445	2878494..2878739	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
GJE03_RS13450	2879150..2879569	-	420	WP_003413190.1	A24 family peptidase	-
GJE03_RS13455	2879581..2880390	-	810	WP_003413193.1	shikimate dehydrogenase	-
GJE03_RS13460	2880387..2881640	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
GJE03_RS13465	2881633..2882145	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (3-111)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 13728.72 Da
• Isoelectric Point: 4.4687

>T143148 WP_003413174.1 NZ_CP045962:2877235-2877612 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Nucleotide

• Length: 378 bp

>T143148 NZ_CP060953:c213759-213481 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT143148 WP_003413167.1 NZ_CP045962:2876981-2877238 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT143148 NZ_CP060953:c213481-213197 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: G0TQ28

Antitoxin

• AlphaFold DB: A0A829C9W5