Detailed information of TA system TA143146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2861986..2862626
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ08
• Locus tag: GJE03_RS13330
• Protein ID: WP_003412970.1
• Coordinates: 2861986..2862405 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ09
• Locus tag: GJE03_RS13335
• Protein ID: WP_003412975.1
• Coordinates: 2862402..2862626 (-)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS13305	2857571..2858293	-	723	WP_003412957.1	DUF1906 domain-containing protein	-
GJE03_RS13310	2858810..2859037	+	228	WP_003412960.1	type II toxin-antitoxin system VapB family antitoxin	-
GJE03_RS13315	2859034..2859435	+	402	WP_003412963.1	PIN domain-containing protein	-
GJE03_RS13320	2859470..2860390	-	921	WP_003412965.1	restriction endonuclease	-
GJE03_RS20850	2860731..2860976	-	246	WP_071854223.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
GJE03_RS13325	2861035..2861985	+	951	WP_003911916.1	Lsr2 family protein	-
GJE03_RS13330	2861986..2862405	-	420	WP_003412970.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJE03_RS13335	2862402..2862626	-	225	WP_003412975.1	antitoxin VapB39	Antitoxin
GJE03_RS13340	2862657..2865500	-	2844	WP_003899363.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
GJE03_RS13345	2865572..2865973	-	402	WP_003412981.1	hypothetical protein	-
GJE03_RS13350	2865973..2866443	-	471	WP_003412985.1	transcription antitermination factor NusB	-
GJE03_RS13355	2866446..2867009	-	564	WP_003412989.1	elongation factor P	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-128)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14732.70 Da
• Isoelectric Point: 6.7519

>T143146 WP_003412970.1 NZ_CP045962:c2862405-2861986 [Mycobacterium tuberculosis]
VTALLDVNVLIALGWPNHVHHAAAQRWFTQFSSNGWATTPITEAGYVRISSNRSVMQVSTTPAIAIAQLAAMTSLAGHTF
WPDDVPLIVGSAGDRDAVSNHRRVTDCHLIALAARYGGRLVTFDAALADSASAGLVEVL

Nucleotide

• Length: 420 bp

>T143146 NZ_CP060951:2269-2544 [Escherichia coli]
ATGGAACTGAAGTGGACCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTGGCCAGTAAACCTGC
GGCCGCCAGAACGGTGCAGTCCCTGACACAGGCTCCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTACTGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 7993.94 Da
• Isoelectric Point: 4.0057

>AT143146 WP_003412975.1 NZ_CP045962:c2862626-2862402 [Mycobacterium tuberculosis]
MRTTLQIDDDVLEDARSIARSEGKSVGAVISELARRSLRPVGIVEVDGFPVFDVPPDAPTVTSEDVVRALEDDV

Nucleotide

• Length: 225 bp

>AT143146 NZ_CP060951:1985-2269 [Escherichia coli]
ATGCAAATGAAAAACAATACCGCACAGGCAACAAAAGTAATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGCTGGATTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATCGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: G0TQ08

Antitoxin

• AlphaFold DB: G0TQ09