Detailed information of TA system TA143134

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2195537..2196240
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: GJE03_RS10140
• Protein ID: WP_003409778.1
• Coordinates: 2195537..2195866 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: GJE03_RS10145
• Protein ID: WP_003409780.1
• Coordinates: 2195863..2196240 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS10120	2191920..2192990	+	1071	WP_154079956.1	epoxide hydrolase EphB	-
GJE03_RS10125	2192987..2193502	+	516	WP_003409718.1	flavin reductase family protein	-
GJE03_RS10130	2193499..2194560	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
GJE03_RS10135	2194557..2195327	+	771	WP_003409775.1	SDR family oxidoreductase	-
GJE03_RS10140	2195537..2195866	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
GJE03_RS10145	2195863..2196240	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
GJE03_RS10150	2196237..2196827	-	591	WP_003409784.1	SEC-C domain-containing protein	-
GJE03_RS10155	2196882..2198246	+	1365	WP_003903691.1	HNH endonuclease	-
GJE03_RS10160	2198401..2198853	-	453	WP_003899095.1	lipoprotein	-
GJE03_RS10165	2198917..2199318	+	402	WP_003409869.1	hypothetical protein	-
GJE03_RS10170	2199311..2199493	-	183	WP_003409870.1	hypothetical protein	-
GJE03_RS10175	2199607..2199957	-	351	WP_003409871.1	hypothetical protein	-
GJE03_RS10180	2199968..2200870	-	903	WP_003409874.1	hypothetical protein	-
GJE03_RS10185	2200891..2201082	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T143134 WP_003409778.1 NZ_CP045962:c2195866-2195537 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T143134 NZ_CP060950:2530073-2530423 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT143134 WP_003409780.1 NZ_CP045962:c2196240-2195863 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT143134 NZ_CP060950:2529828-2530079 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8