Detailed information of TA system TA143125

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1239196..1239764
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TH08
• Locus tag: GJE03_RS05910
• Protein ID: WP_003405865.1
• Coordinates: 1239390..1239764 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TH07
• Locus tag: GJE03_RS05905
• Protein ID: WP_003405863.1
• Coordinates: 1239196..1239393 (+)
• Length: 66 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS05885	1235237..1235875	-	639	WP_003898730.1	lipid droplet-associated protein	-
GJE03_RS05890	1235965..1236972	+	1008	WP_003405853.1	4-hydroxy-3-methylbut-2-enyl diphosphate reductase	-
GJE03_RS05895	1236989..1237972	-	984	WP_003903326.1	hypothetical protein	-
GJE03_RS05900	1238035..1239108	+	1074	WP_003898732.1	redox-regulated ATPase YchF	-
GJE03_RS05905	1239196..1239393	+	198	WP_003405863.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
GJE03_RS05910	1239390..1239764	+	375	WP_003405865.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJE03_RS05915	1239967..1240665	+	699	WP_003898733.1	hypothetical protein	-
GJE03_RS05920	1240783..1240968	+	186	WP_003901093.1	hypothetical protein	-
GJE03_RS05925	1240895..1241170	-	276	WP_003405867.1	hypothetical protein	-
GJE03_RS05930	1241413..1241736	+	324	WP_003405871.1	antibiotic biosynthesis monooxygenase	-
GJE03_RS05935	1241751..1242461	-	711	WP_003911399.1	hypothetical protein	-
GJE03_RS20725	1242483..1242692	+	210	WP_003911400.1	hypothetical protein	-
GJE03_RS05940	1242644..1243351	-	708	Protein_1159	adenylate/guanylate cyclase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-111)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (1-42)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13444.56 Da
• Isoelectric Point: 5.1881

>T143125 WP_003405865.1 NZ_CP045962:1239390-1239764 [Mycobacterium tuberculosis]
VILVDTSVWIEHLRAADARLVELLGDDEAGCHPLVIEELALGSIKQRDVVLDLLANLYQFPVVTHDEVLRLVGRRRLWGR
GLGAVDANLLGSVALVGGARLWTRDKRLKAACAESGVALAEEVS

Nucleotide

• Length: 375 bp

>T143125 NZ_CP060950:c908697-908362 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 66 a.a.
• Molecular weight: 6973.03 Da
• Isoelectric Point: 11.7327

>AT143125 WP_003405863.1 NZ_CP045962:1239196-1239393 [Mycobacterium tuberculosis]
MRTTVTVDDALLAKAAELTGVKEKSTLLREGLQTLVRVESARRLAALGGTDPQATAAPRRRTSPR

Nucleotide

• Length: 198 bp

>AT143125 NZ_CP060950:c908945-908697 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• AlphaFold DB: G0TH08

Antitoxin

• AlphaFold DB: G0TH07