Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1230440..1231071 Replicon chromosome
Accession NZ_CP045962
Organism Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag GJE03_RS05855 Protein ID WP_003405820.1
Coordinates 1230440..1230751 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag GJE03_RS05860 Protein ID WP_003405836.1
Coordinates 1230751..1231071 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE03_RS05835 1225921..1227345 - 1425 WP_003405805.1 class II fumarate hydratase -
GJE03_RS05840 1227376..1228464 - 1089 WP_017351234.1 class II fructose-bisphosphatase -
GJE03_RS05845 1228520..1229164 + 645 WP_003915494.1 DUF4245 domain-containing protein -
GJE03_RS05850 1229171..1230328 - 1158 WP_003898727.1 AI-2E family transporter -
GJE03_RS05855 1230440..1230751 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
GJE03_RS05860 1230751..1231071 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
GJE03_RS05865 1231081..1232606 + 1526 Protein_1143 carboxylesterase/lipase family protein -
GJE03_RS05870 1232624..1233736 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
GJE03_RS05875 1233746..1234003 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
GJE03_RS05880 1233993..1235240 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
GJE03_RS05885 1235237..1235875 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T143124 WP_003405820.1 NZ_CP045962:c1230751-1230440 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T143124 NZ_CP060950:c573949-573551 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTGATTGAAGGTTTCGTATCTCGTTTGGAAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGATTGGAGCTAA

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT143124 WP_003405836.1 NZ_CP045962:c1231071-1230751 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT143124 NZ_CP060950:c574176-573949 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References