Detailed information of TA system TA143111

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 700933..701579
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: GJE03_RS03135
• Protein ID: WP_003403137.1
• Coordinates: 700933..701346 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: GJE03_RS03140
• Protein ID: WP_003403139.1
• Coordinates: 701343..701579 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS03115	697016..698566	+	1551	WP_003403119.1	MCE family protein	-
GJE03_RS03120	698618..699010	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
GJE03_RS03125	699007..699264	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
GJE03_RS03130	699447..700682	-	1236	WP_003403128.1	ATP-binding protein	-
GJE03_RS03135	700933..701346	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
GJE03_RS03140	701343..701579	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GJE03_RS03145	701683..702189	-	507	WP_003900973.1	two component system sensor kinase HK1	-
GJE03_RS03150	702303..702833	-	531	WP_003917320.1	two component system sensor kinase HK2	-
GJE03_RS03155	702817..703578	-	762	WP_003403148.1	two-component system response regulator TcrA	-
GJE03_RS03160	703635..703946	+	312	WP_003403164.1	hypothetical protein	-
GJE03_RS03165	704018..704968	+	951	WP_003907436.1	DUF1259 domain-containing protein	-
GJE03_RS03170	705209..705793	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
GJE03_RS03175	705795..706505	+	711	Protein_626	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T143111 WP_003403137.1 NZ_CP045962:c701346-700933 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T143111 NZ_CP060946:3495470-3495643 [Escherichia coli O157:H7 str. EC10]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTAGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT143111 WP_003403139.1 NZ_CP045962:c701579-701343 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT143111 NZ_CP060946:3495146-3495442 [Escherichia coli O157:H7 str. EC10]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89