Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN-CcdA
Location 643980..644656 Replicon chromosome
Accession NZ_CP045962
Organism Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)


Gene name vapC Uniprot ID G0TPQ9
Locus tag GJE03_RS02880 Protein ID WP_003898500.1
Coordinates 643980..644393 (-) Length 138 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TPR0
Locus tag GJE03_RS02885 Protein ID WP_003402915.1
Coordinates 644390..644656 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE03_RS02850 639325..639627 - 303 WP_003402896.1 DUF3349 domain-containing protein -
GJE03_RS02855 639687..639965 - 279 WP_003402901.1 hypothetical protein -
GJE03_RS02860 639962..641215 - 1254 WP_003402904.1 inorganic phosphate transporter family protein -
GJE03_RS02865 641335..641721 - 387 WP_003402907.1 VOC family protein -
GJE03_RS02870 641784..642668 - 885 WP_003402909.1 SDR family oxidoreductase -
GJE03_RS02875 642764..643666 - 903 WP_003915439.1 1,4-dihydroxy-2-naphthoyl-CoA synthase -
GJE03_RS02880 643980..644393 - 414 WP_003898500.1 PIN domain-containing protein Toxin
GJE03_RS02885 644390..644656 - 267 WP_003402915.1 antitoxin VapB3 Antitoxin
GJE03_RS02890 644848..646563 - 1716 WP_003402917.1 fatty-acid--CoA ligase FadD8 -
GJE03_RS02895 646641..648245 + 1605 WP_003903056.1 amidohydrolase -
GJE03_RS02900 648242..649222 + 981 WP_003402923.1 o-succinylbenzoate synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Pae0151-like, PIN domain

(13-129)

Antitoxin

Post-segregation antitoxin CcdA

(19-61)


Sequences

Toxin        


Download         Length: 138 a.a.        Molecular weight: 14667.78 Da        Isoelectric Point: 7.3603

>T143108 WP_003898500.1 NZ_CP045962:c644393-643980 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Download         Length: 414 bp

>T143108 NZ_CP060946:c2816677-2816570 [Escherichia coli O157:H7 str. EC10]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9543.79 Da        Isoelectric Point: 4.5994

>AT143108 WP_003402915.1 NZ_CP045962:c644656-644390 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Download         Length: 267 bp

>AT143108 NZ_CP060946:2816734-2816789 [Escherichia coli O157:H7 str. EC10]
TCAAGAATGGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPQ9

Antitoxin

Source ID Structure
AlphaFold DB G0TPR0

References