Detailed information of TA system TA143105

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 366968..367539
• Replicon: chromosome
• Accession: NZ_CP045962
• Organism: Mycobacterium tuberculosis strain AUSMDU00018547

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: GJE03_RS01595
• Protein ID: WP_003401560.1
• Coordinates: 366968..367270 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: GJE03_RS01600
• Protein ID: WP_003401563.1
• Coordinates: 367318..367539 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE03_RS01575	362392..363195	-	804	WP_003401540.1	hypothetical protein	-
GJE03_RS01580	363205..364602	-	1398	WP_003401544.1	sulfatase	-
GJE03_RS01585	364781..366601	+	1821	WP_031653699.1	PE family protein	-
GJE03_RS01590	366744..366971	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
GJE03_RS01595	366968..367270	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
GJE03_RS01600	367318..367539	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
GJE03_RS01605	367536..367961	+	426	WP_003401566.1	PIN domain nuclease	-
GJE03_RS01610	368097..368729	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
GJE03_RS01615	368726..369634	+	909	WP_154079877.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T143105 WP_003401560.1 NZ_CP045962:366968-367270 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T143105 NZ_CP060946:2409398-2409862 [Escherichia coli O157:H7 str. EC10]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT143105 WP_003401563.1 NZ_CP045962:367318-367539 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT143105 NZ_CP060946:2409063-2409398 [Escherichia coli O157:H7 str. EC10]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A829CG48