Detailed information of TA system TA143087

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 197704..198229
• Replicon: plasmid pAUSMDU00010535_01
• Accession: NZ_CP045942
• Organism: Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: Q326Z8
• Locus tag: GJE14_RS24845
• Protein ID: WP_001159860.1
• Coordinates: 197924..198229 (+)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: Q7BEK0
• Locus tag: GJE14_RS24840
• Protein ID: WP_000813626.1
• Coordinates: 197704..197922 (+)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE14_RS24805 (GJE14_24805)	193430..193621	+	192	Protein_228	ATP-binding protein	-
GJE14_RS24810 (GJE14_24810)	193715..193924	-	210	Protein_229	peptidoglycan-binding protein	-
GJE14_RS24815 (GJE14_24815)	193974..194225	-	252	WP_001381727.1	transporter	-
GJE14_RS24820 (GJE14_24820)	194677..194961	+	285	WP_011114774.1	ribbon-helix-helix domain-containing protein	-
GJE14_RS24825 (GJE14_24825)	194961..195236	+	276	WP_000421262.1	type II toxin-antitoxin system RelE/ParE family toxin	-
GJE14_RS24835 (GJE14_24835)	196215..197168	+	954	Protein_234	IS66 family transposase	-
GJE14_RS24840 (GJE14_24840)	197704..197922	+	219	WP_000813626.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
GJE14_RS24845 (GJE14_24845)	197924..198229	+	306	WP_001159860.1	type II toxin-antitoxin system toxin CcdB	Toxin
GJE14_RS24855 (GJE14_24855)	198622..200259	+	1638	WP_000936806.1	T3SS effector E3 ubiquitin-protein ligase IpaH9.8	-
GJE14_RS24860 (GJE14_24860)	200467..200736	+	270	Protein_238	type II toxin-antitoxin system antitoxin YacA	-
GJE14_RS26510	200736..200905	+	170	Protein_239	type II toxin-antitoxin system toxin YacB	-
GJE14_RS24865 (GJE14_24865)	200988..201578	+	591	WP_000705601.1	type III secretion system effector protein kinase OspG	-
GJE14_RS25915	201935..202201	+	267	Protein_241	IS66 family insertion sequence element accessory protein TnpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	ipaH1.4 / ospE2 / nleE / icsP/sopA / ospB / ospC3 / ospD2 / ospF / ospD1 / ipgB2 / ospE2 / ipaH2.5 / ospC2 / ipaH7.8 / ipaH4.5 / ospD3/senA / ospC1 / ospC3 / ipaJ / ipaA / ipaD / ipaC / ipaB / ipgC / ipgB1 / ipgA / icsB / ipgD / ipgE / ipgF / mxiG / mxiH / mxiI / mxiJ / mxiK / mxiN / mxiL / mxiM / mxiE / mxiD / mxiC / mxiA / spa15 / spa47 / spa13 / spa32 / spa33 / spa24 / spa9 / spa29 / spa40 / virA / icsA/virG / papC / ospI / ipaH9.8 / ospG	1..234182	234182
	-	inside	IScluster/Tn	-	ospI / ipaH9.8 / ospG	182928..207068	24140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11736.59 Da
• Isoelectric Point: 6.4674

>T143087 WP_001159860.1 NZ_CP045942:197924-198229 [Shigella flexneri 2a]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHVGDESWRMMTTDMASVPIFVIGEEV
ADLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T143087 NZ_CP060938:3327395-3327502 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8333.36 Da
• Isoelectric Point: 6.2838

>AT143087 WP_000813626.1 NZ_CP045942:197704-197922 [Shigella flexneri 2a]
MKQRITVTIDSDSYQLLKSANVNISGLVNTAMQKEARRLRAERWQAENQQGMAEIARFIEMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT143087 NZ_CP060938:c3327348-3327282 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Structures

Toxin

• AlphaFold DB: A0A4P7TTN3

Antitoxin

• AlphaFold DB: Q7BEK0