Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 869731..870532 Replicon chromosome
Accession NZ_CP045941
Organism Shigella flexneri 2a strain AUSMDU00010535

Toxin (Protein)


Gene name yeeV Uniprot ID D3GUT5
Locus tag GJE14_RS04295 Protein ID WP_001094430.1
Coordinates 869731..870108 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID D3GUT4
Locus tag GJE14_RS04300 Protein ID WP_001285620.1
Coordinates 870155..870532 (-) Length 126 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE14_RS04265 (864944) 864944..865411 + 468 WP_011069513.1 prepilin peptidase -
GJE14_RS04275 (867080) 867080..867325 - 246 WP_000875412.1 hypothetical protein -
GJE14_RS04280 (868102) 868102..868944 - 843 WP_001290187.1 DUF4942 domain-containing protein -
GJE14_RS04285 (869029) 869029..869226 - 198 WP_000772029.1 DUF957 domain-containing protein -
GJE14_RS04290 (869246) 869246..869734 - 489 WP_000761715.1 DUF5983 family protein -
GJE14_RS04295 (869731) 869731..870108 - 378 WP_001094430.1 TA system toxin CbtA family protein Toxin
GJE14_RS04300 (870155) 870155..870532 - 378 WP_001285620.1 type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA Antitoxin
GJE14_RS04305 (870612) 870612..870833 - 222 WP_000692350.1 DUF987 domain-containing protein -
GJE14_RS04310 (870920) 870920..871396 - 477 WP_128570583.1 RadC family protein -
GJE14_RS04315 (871412) 871412..871885 - 474 WP_000855059.1 antirestriction protein -
GJE14_RS04320 (872227) 872227..873045 - 819 WP_001177758.1 DUF932 domain-containing protein -
GJE14_RS26030 (873224) 873224..873313 - 90 WP_112031555.1 DUF905 family protein -
GJE14_RS04330 (873456) 873456..873911 - 456 WP_005051844.1 IrmA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13971.82 Da        Isoelectric Point: 6.8608

>T143065 WP_001094430.1 NZ_CP045941:c870108-869731 [Shigella flexneri 2a]
MNTLPDTHVREASGCPSPITIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRDNYRTVNNITRGKHPEAKQ

Download         Length: 378 bp

>T143065 NZ_CP060933:3798010-3798228 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 126 a.a.        Molecular weight: 13698.52 Da        Isoelectric Point: 5.9505

>AT143065 WP_001285620.1 NZ_CP045941:c870532-870155 [Shigella flexneri 2a]
VSDTLPGTTPPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDVDAYHLDQAFPLLMKQLELMLTGG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAAPATTV

Download         Length: 378 bp

>AT143065 NZ_CP060933:3797610-3797984 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y6M7

Antitoxin

Source ID Structure
PDB 2INW
AlphaFold DB A0A0E0Y522

References