Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4085268..4085765 Replicon chromosome
Accession NZ_CP045932
Organism Shigella sonnei strain AUSMDU00010534

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag GJE15_RS20540 Protein ID WP_001173343.1
Coordinates 4085268..4085441 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag GJE15_RS20545 Protein ID WP_000398619.1
Coordinates 4085469..4085765 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE15_RS20520 (4080420) 4080420..4081576 + 1157 WP_087879933.1 IS3-like element IS600 family transposase -
GJE15_RS20525 (4081622) 4081622..4081879 + 258 Protein_4083 lysine decarboxylase LdcC -
GJE15_RS20530 (4081938) 4081938..4083395 + 1458 WP_000856831.1 dipeptide/tripeptide permease DtpC -
GJE15_RS20535 (4083632) 4083632..4085149 + 1518 WP_001295086.1 lysine--tRNA ligase -
GJE15_RS20540 (4085268) 4085268..4085441 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
GJE15_RS20545 (4085469) 4085469..4085765 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
GJE15_RS20550 (4085992) 4085992..4086264 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
GJE15_RS20555 (4086276) 4086276..4086506 - 231 WP_000371698.1 4Fe-4S mono-cluster protein YjdI -
GJE15_RS20560 (4086687) 4086687..4088318 + 1632 WP_001216495.1 sensor histidine kinase -
GJE15_RS20565 (4088315) 4088315..4089034 + 720 WP_000611285.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T143056 WP_001173343.1 NZ_CP045932:c4085441-4085268 [Shigella sonnei]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T143056 NZ_CP060933:1639823-1640221 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT143056 WP_000398619.1 NZ_CP045932:c4085765-4085469 [Shigella sonnei]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT143056 NZ_CP060933:1639596-1639823 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References