Detailed information of TA system TA143054

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): itaRT/DUF1778(antitoxin)
• Location: 3396697..3397534
• Replicon: chromosome
• Accession: NZ_CP045932
• Organism: Shigella sonnei strain AUSMDU00010534

Toxin (Protein)

• Gene name: itaT
• Uniprot ID: Q3Z4X7
• Locus tag: GJE15_RS17220
• Protein ID: WP_000227784.1
• Coordinates: 3396992..3397534 (+)
• Length: 181 a.a.

Antitoxin (Protein)

• Gene name: itaR
• Uniprot ID: I2UQS9
• Locus tag: GJE15_RS17215
• Protein ID: WP_001297137.1
• Coordinates: 3396697..3397008 (+)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GJE15_RS17190 (3391717)	3391717..3392664	+	948	WP_001239441.1	cytochrome o ubiquinol oxidase subunit II	-
GJE15_RS17195 (3392686)	3392686..3394677	+	1992	WP_000467180.1	cytochrome o ubiquinol oxidase subunit I	-
GJE15_RS17200 (3394667)	3394667..3395281	+	615	WP_000179819.1	cytochrome o ubiquinol oxidase subunit III	-
GJE15_RS17205 (3395281)	3395281..3395610	+	330	WP_000019869.1	cytochrome o ubiquinol oxidase subunit IV	-
GJE15_RS17210 (3395622)	3395622..3396512	+	891	WP_000971327.1	heme o synthase	-
GJE15_RS17215 (3396697)	3396697..3397008	+	312	WP_001297137.1	DUF1778 domain-containing protein	Antitoxin
GJE15_RS17220 (3396992)	3396992..3397534	+	543	WP_000227784.1	GNAT family N-acetyltransferase	Toxin
GJE15_RS17225 (3397590)	3397590..3398156	-	567	Protein_3422	SEL1-like repeat protein	-
GJE15_RS17230 (3398190)	3398190..3398887	+	698	WP_094096600.1	IS1-like element IS1A family transposase	-
GJE15_RS17235 (3398904)	3398904..3399302	-	399	Protein_3424	SEL1-like repeat protein	-
GJE15_RS17245 (3400487)	3400487..3401851	+	1365	WP_001000971.1	MFS transporter	-
GJE15_RS17250 (3401979)	3401979..3402470	-	492	WP_001138904.1	nucleotide binding protein YajQ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (68-156)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-97)

Sequences

Toxin

Protein

• Length: 181 a.a.
• Molecular weight: 19805.02 Da
• Isoelectric Point: 8.3395

>T143054 WP_000227784.1 NZ_CP045932:3396992-3397534 [Shigella sonnei]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA

Nucleotide

• Length: 543 bp

>T143054 NZ_CP060933:1187664-1188071 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGTGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11358.04 Da
• Isoelectric Point: 10.4825

>AT143054 WP_001297137.1 NZ_CP045932:3396697-3397008 [Shigella sonnei]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA

Nucleotide

• Length: 312 bp

>AT143054 NZ_CP060933:1187417-1187683 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 7BYY

Antitoxin

• AlphaFold DB: A0A0E0Y6B3