Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3359221..3359839 Replicon chromosome
Accession NZ_CP045932
Organism Shigella sonnei strain AUSMDU00010534

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag GJE15_RS17030 Protein ID WP_001291435.1
Coordinates 3359621..3359839 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0H8V4S8
Locus tag GJE15_RS17025 Protein ID WP_000344804.1
Coordinates 3359221..3359595 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE15_RS17015 (3354310) 3354310..3355503 + 1194 WP_011310152.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
GJE15_RS17020 (3355526) 3355526..3358675 + 3150 WP_001132464.1 efflux RND transporter permease AcrB -
GJE15_RS17025 (3359221) 3359221..3359595 + 375 WP_000344804.1 Hha toxicity modulator TomB Antitoxin
GJE15_RS17030 (3359621) 3359621..3359839 + 219 WP_001291435.1 HHA domain-containing protein Toxin
GJE15_RS17035 (3360010) 3360010..3360561 + 552 WP_000102564.1 maltose O-acetyltransferase -
GJE15_RS17040 (3360677) 3360677..3361147 + 471 WP_052942789.1 YlaC family protein -
GJE15_RS17045 (3361311) 3361311..3362859 + 1549 Protein_3386 cyclic-guanylate-specific phosphodiesterase PdeB -
GJE15_RS17050 (3362901) 3362901..3363254 - 354 WP_000878140.1 DUF1428 domain-containing protein -
GJE15_RS17060 (3363633) 3363633..3363944 + 312 WP_000409911.1 MGMT family protein -
GJE15_RS17065 (3363975) 3363975..3364547 - 573 WP_000779812.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T143053 WP_001291435.1 NZ_CP045932:3359621-3359839 [Shigella sonnei]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T143053 NZ_CP060933:c1038748-1038371 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGCGATGCGGTGAACTTTCTCGTTGAAAAATACGCACTGGTACGTACCGACCAGCCAGGATTC
AGCGCCTGTACCCGTTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCCCGCCGGGCAACCGGCCTGATGGCCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAACATCCGGGGGCGAAACAATGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14615.43 Da        Isoelectric Point: 4.6232

>AT143053 WP_000344804.1 NZ_CP045932:3359221-3359595 [Shigella sonnei]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIETFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT143053 NZ_CP060933:c1039169-1038795 [Escherichia coli]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAGGGCTGACCTGCAAAGCCGATACCCTCAGCAGTTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H8V4S8

References