Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 614558..615212 Replicon chromosome
Accession NZ_CP045932
Organism Shigella sonnei strain AUSMDU00010534

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag GJE15_RS03175 Protein ID WP_000244777.1
Coordinates 614805..615212 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag GJE15_RS03170 Protein ID WP_000354046.1
Coordinates 614558..614824 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE15_RS03145 (609727) 609727..610470 + 744 WP_000951937.1 SDR family oxidoreductase -
GJE15_RS03150 (610527) 610527..611960 - 1434 WP_005137189.1 6-phospho-beta-glucosidase BglA -
GJE15_RS03155 (612005) 612005..612316 + 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
GJE15_RS03160 (612480) 612480..613139 + 660 WP_000250274.1 hemolysin III family protein -
GJE15_RS03165 (613335) 613335..614315 - 981 WP_000886060.1 tRNA-modifying protein YgfZ -
GJE15_RS03170 (614558) 614558..614824 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
GJE15_RS03175 (614805) 614805..615212 + 408 WP_000244777.1 protein YgfX Toxin
GJE15_RS03180 (615252) 615252..615773 - 522 WP_001055874.1 flavodoxin FldB -
GJE15_RS03185 (615885) 615885..616781 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
GJE15_RS03190 (616806) 616806..617516 + 711 WP_000715206.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
GJE15_RS03195 (617522) 617522..619255 + 1734 WP_000813167.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T143040 WP_000244777.1 NZ_CP045932:614805-615212 [Shigella sonnei]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T143040 NZ_CP060928:c2751858-2751751 [Escherichia coli]
ATGACGTTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT143040 WP_000354046.1 NZ_CP045932:614558-614824 [Shigella sonnei]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT143040 NZ_CP060928:2751906-2751972 [Escherichia coli]
GGTCTAGAGTCAAGATTAGCCCCCGTGGTGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References