Detailed information of TA system TA143035

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 4753760..4754406
• Replicon: chromosome
• Accession: NZ_CP045929
• Organism: Saccharopolyspora coralli strain E2A

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GIY23_RS22225
• Protein ID: WP_154079061.1
• Coordinates: 4753993..4754406 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GIY23_RS22220
• Protein ID: WP_154078428.1
• Coordinates: 4753760..4753975 (+)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY23_RS22205	4750149..4750892	+	744	WP_154078425.1	MerR family transcriptional regulator	-
GIY23_RS22210	4751038..4752228	+	1191	WP_154078426.1	ATP-binding protein	-
GIY23_RS22215	4752882..4753598	-	717	WP_154078427.1	hypothetical protein	-
GIY23_RS22220	4753760..4753975	+	216	WP_154078428.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
GIY23_RS22225	4753993..4754406	+	414	WP_154079061.1	PIN domain-containing protein	Toxin
GIY23_RS22230	4754452..4756308	+	1857	WP_154078429.1	NHL domain-containing thioredoxin family protein	-
GIY23_RS22235	4756380..4756700	+	321	WP_154078430.1	co-chaperone GroES	-
GIY23_RS22240	4757368..4759011	+	1644	WP_154079062.1	VanW family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (2-121)

Antitoxin

• Post-segregation antitoxin CcdA (3-45)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14780.04 Da
• Isoelectric Point: 6.2148

>T143035 WP_154079061.1 NZ_CP045929:4753993-4754406 [Saccharopolyspora coralli]
VVLDASVAVDLLTGTDCAPAASARLAGTELHAPAHLDAEVLSALGRMQRAGDLAAVDVELAIDRLAVMPVTRHPLPNLLT
GAWARRAYVRLLDGLYIALAEHLGLRVLTTDLRLARACPELTDTLHRPTEQRDVRHE

Nucleotide

• Length: 414 bp

>T143035 NZ_CP060928:c1976653-1976318 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTCG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7676.69 Da
• Isoelectric Point: 4.9681

>AT143035 WP_154078428.1 NZ_CP045929:4753760-4753975 [Saccharopolyspora coralli]
MTRMNVWVPDDLAARAREARLNVSALTQAAIAAELARTATDAWLATLPAPRGLTHEASMRALDDAREEFGA

Nucleotide

• Length: 216 bp

>AT143035 NZ_CP060928:c1976901-1976653 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGACTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA