Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1633843..1634367 Replicon chromosome
Accession NZ_CP045927
Organism Staphylococcus agnetis strain 1379

Toxin (Protein)


Gene name mazF Uniprot ID A0A0A8HRS0
Locus tag GJE18_RS08320 Protein ID WP_037567539.1
Coordinates 1633843..1634196 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A242VEK4
Locus tag GJE18_RS08325 Protein ID WP_060551514.1
Coordinates 1634197..1634367 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GJE18_RS08300 1631145..1631915 - 771 WP_060551512.1 RNA polymerase sigma factor SigB -
GJE18_RS08305 1631890..1632369 - 480 WP_037567547.1 anti-sigma B factor RsbW -
GJE18_RS08310 1632372..1632698 - 327 WP_037567545.1 anti-sigma factor antagonist -
GJE18_RS08315 1632779..1633783 - 1005 WP_060551513.1 PP2C family protein-serine/threonine phosphatase -
GJE18_RS08320 1633843..1634196 - 354 WP_037567539.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GJE18_RS08325 1634197..1634367 - 171 WP_060551514.1 hypothetical protein Antitoxin
GJE18_RS08330 1634452..1635600 - 1149 WP_165886133.1 alanine racemase -
GJE18_RS08335 1635706..1636056 - 351 WP_060551516.1 holo-ACP synthase -
GJE18_RS08340 1636128..1636634 - 507 WP_157951749.1 PH domain-containing protein -
GJE18_RS08345 1636618..1638105 - 1488 WP_060551518.1 PH domain-containing protein -
GJE18_RS08350 1638098..1638574 - 477 WP_060551519.1 PH domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-111)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13103.33 Da        Isoelectric Point: 10.3729

>T143028 WP_037567539.1 NZ_CP045927:c1634196-1633843 [Staphylococcus agnetis]
MKRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKHKYKLDKDSVILLEQIRT
VDKKRLKEKLTYLSDKKMKEVNAALGISLGLHMNQHK

Download         Length: 354 bp

>T143028 NZ_CP060928:561314-561490 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATAGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6277.94 Da        Isoelectric Point: 4.0644

>AT143028 WP_060551514.1 NZ_CP045927:c1634367-1634197 [Staphylococcus agnetis]
MSAFYQIRGKSLEQSLKEGYVQMADLNLSLATEAYSVECEACDCNETHLLSNNKDD

Download         Length: 171 bp

>AT143028 NZ_CP060928:561536-561952 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
CCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCACGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A8HRS0

Antitoxin

Source ID Structure
AlphaFold DB A0A242VEK4

References