Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3640026..3640663 Replicon chromosome
Accession NZ_CP045926
Organism Bacillus velezensis strain AL7

Toxin (Protein)


Gene name mazF Uniprot ID G4NU33
Locus tag GI367_RS17755 Protein ID WP_003156187.1
Coordinates 3640313..3640663 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID I2HMS5
Locus tag GI367_RS17750 Protein ID WP_003156188.1
Coordinates 3640026..3640307 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GI367_RS17730 3636391..3636990 - 600 WP_007609579.1 rhomboid family intramembrane serine protease -
GI367_RS17735 3637083..3637448 + 366 WP_007609580.1 holo-ACP synthase -
GI367_RS17740 3637613..3638620 + 1008 WP_007410230.1 outer membrane lipoprotein carrier protein LolA -
GI367_RS17745 3638737..3639906 + 1170 WP_007609581.1 alanine racemase -
GI367_RS17750 3640026..3640307 + 282 WP_003156188.1 type II toxin-antitoxin system antitoxin EndoAI Antitoxin
GI367_RS17755 3640313..3640663 + 351 WP_003156187.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
GI367_RS17760 3640781..3641602 + 822 WP_003156182.1 STAS domain-containing protein -
GI367_RS17765 3641607..3641972 + 366 WP_007609584.1 RsbT antagonist protein RsbS -
GI367_RS17770 3641975..3642376 + 402 WP_003156178.1 anti-sigma regulatory factor -
GI367_RS17775 3642388..3643395 + 1008 WP_007609589.1 PP2C family protein-serine/threonine phosphatase -
GI367_RS17780 3643459..3643788 + 330 WP_003156176.1 anti-sigma factor antagonist RsbV -
GI367_RS17785 3643785..3644267 + 483 WP_007609591.1 anti-sigma B factor RsbW -
GI367_RS17790 3644233..3645021 + 789 WP_003156171.1 RNA polymerase sigma factor SigB -
GI367_RS17795 3645021..3645623 + 603 WP_007410234.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(12-45)


Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12977.98 Da        Isoelectric Point: 4.8781

>T143026 WP_003156187.1 NZ_CP045926:3640313-3640663 [Bacillus velezensis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNDIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMDKVDEALQISLALIDF

Download         Length: 351 bp

>T143026 NZ_CP060928:c347817-347710 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGACTTTCTGGCACGACCTGGCGGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCGGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10562.92 Da        Isoelectric Point: 6.4706

>AT143026 WP_003156188.1 NZ_CP045926:3640026-3640307 [Bacillus velezensis]
MSESSARTEMKISLPENLVAELDGVAMREKRSRNELISQAVRAYVSERTTRHNRDLMRRGYMEMAKINLNISSEAHYAEC
EAETTVERLVSGG

Download         Length: 282 bp

>AT143026 NZ_CP060928:347864-347930 [Escherichia coli]
TGTCTGGTTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTTTACCTCTCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4MDX
PDB 4ME7
PDB 1NE8

Antitoxin

Source ID Structure
AlphaFold DB I2HMS5

References