143025

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) spoIISABC/SpoIISA-SpoIISB
Location 487532..488449 Replicon chromosome
Accession NZ_CP045926
Organism Bacillus velezensis strain AL7

Toxin (Protein)


Gene name spoIISA Uniprot ID I2HQ15
Locus tag GI367_RS02590 Protein ID WP_007407256.1
Coordinates 487703..488449 (-) Length 249 a.a.

Antitoxin (Protein)


Gene name spoIISB Uniprot ID I2HQ14
Locus tag GI367_RS02585 Protein ID WP_003154807.1
Coordinates 487532..487702 (-) Length 57 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GI367_RS02550 482756..484387 + 1632 WP_007610826.1 hypothetical protein -
GI367_RS02555 484400..484771 + 372 WP_007610831.1 hypothetical protein -
GI367_RS02560 484776..484973 + 198 WP_007610833.1 XkdX family protein -
GI367_RS02565 485030..485791 + 762 WP_029325867.1 hypothetical protein -
GI367_RS02570 485843..486106 + 264 WP_003154815.1 hemolysin XhlA family protein -
GI367_RS02575 486120..486383 + 264 WP_003154813.1 phage holin -
GI367_RS02580 486397..487275 + 879 WP_007610836.1 N-acetylmuramoyl-L-alanine amidase -
GI367_RS02585 487532..487702 - 171 WP_003154807.1 type II toxin-antitoxin system SpoIISB family antitoxin Antitoxin
GI367_RS02590 487703..488449 - 747 WP_007407256.1 type II toxin-antitoxin system SpoIISA family toxin Toxin
GI367_RS02595 488554..489552 - 999 WP_007610840.1 inorganic phosphate transporter -
GI367_RS02600 489565..490182 - 618 WP_003154804.1 DUF47 domain-containing protein -
GI367_RS02605 490468..491784 - 1317 WP_007610842.1 amino acid permease -
GI367_RS02610 492107..493057 + 951 WP_007610844.1 ring-cleaving dioxygenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 454053..545628 91575


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin SpoIISA, type II toxin-antitoxin system

(3-239)

Antitoxin

Antitoxin SpoIISB

(1-56)


Sequences

Toxin        


Download         Length: 249 a.a.        Molecular weight: 29077.57 Da        Isoelectric Point: 4.7755

>T143025 WP_007407256.1 NZ_CP045926:c488449-487703 [Bacillus velezensis]
MLLFFQIMVWTMAAALILYVYASWRYEAKVKEKMFAIRKTWYLLFVLGSMVYWTYDPESLFAAWRQYLIVAVCFALIDAF
IFLSAYIKKLAGNELETDTREILEENNEMLHSYLEKLKTYQYLLKNEPIHVYYGSTEAYAEGISRLLAAYAEKMNVTASL
CDYSAQSDKDRLTEHMPDAADVQSRLNRKDVYYDQKGRLVLIPFTVQNRHYVIKLTSENLLTEFDYLLFTSLTSIYDLML
PIEEEGDG

Download         Length: 747 bp

>T143025 NZ_CP060928:c347282-347175 [Escherichia coli]
ATGACGCTCGCGCAGTTTGCCATGATTTTCTGGCACGACCTGGCAGCACCGATCCTGGCGGGAATTATTACCGCAGCGAT
TGTCAGCTGGTGGCGTAACCGGAAGTAA

Antitoxin


Download         Length: 57 a.a.        Molecular weight: 6676.74 Da        Isoelectric Point: 10.9273

>AT143025 WP_003154807.1 NZ_CP045926:c487702-487532 [Bacillus velezensis]
MEQAFQNGQPEQRAKPFKMFKKRSMTSIASYQVSPHTARIFKENERLIDKYKQKKA

Download         Length: 171 bp

>AT143025 NZ_CP060928:347330-347396 [Escherichia coli]
GTTCTGGTTCAAGATTAGCCCCCGTTCTGTTGTCAGGTTGTACCTCTCAACGTGCGGGGGTTTTCTC

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB I2HQ15

Antitoxin

Source ID Structure
AlphaFold DB I2HQ14

References