Detailed information of TA system TA143003

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): spoIISABC/SpoIISA-SpoIISB
• Location: 1348032..1348948
• Replicon: chromosome
• Accession: NZ_CP045922
• Organism: Bacillus subtilis strain P8_B1

Toxin (Protein)

• Gene name: spoIISA
• Uniprot ID: O34853
• Locus tag: GII87_RS07085
• Protein ID: WP_003244695.1
• Coordinates: 1348202..1348948 (-)
• Length: 249 a.a.

Antitoxin (Protein)

• Gene name: spoIISB
• Uniprot ID: G4EXD8
• Locus tag: GII87_RS07080
• Protein ID: WP_003232646.1
• Coordinates: 1348032..1348202 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GII87_RS07045	1344895..1345224	+	330	WP_003232660.1	phage-like element PBSX protein XkdW	-
GII87_RS07050	1345221..1345385	+	165	WP_003232658.1	XkdX family protein	-
GII87_RS07055	1345429..1346268	+	840	WP_003245597.1	phage-like element PBSX protein XepA	-
GII87_RS07060	1346321..1346590	+	270	WP_003232655.1	hemolysin XhlA family protein	-
GII87_RS07065	1346603..1346866	+	264	WP_003232653.1	phage holin	-
GII87_RS07070	1346879..1347772	+	894	WP_003245230.1	N-acetylmuramoyl-L-alanine amidase	-
GII87_RS07075	1347809..1347946	-	138	WP_003232648.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISC	-
GII87_RS07080	1348032..1348202	-	171	WP_003232646.1	three component toxin-antitoxin-antitoxin system antitoxin SpoIISB	Antitoxin
GII87_RS07085	1348202..1348948	-	747	WP_003244695.1	toxin-antitoxin-antitoxin system toxin SpoIISA	Toxin
GII87_RS07090	1349058..1350059	-	1002	WP_003232642.1	inorganic phosphate transporter	-
GII87_RS07095	1350072..1350689	-	618	WP_003218470.1	DUF47 domain-containing protein	-
GII87_RS07100	1350965..1352281	-	1317	WP_003244819.1	serine/threonine exchanger	-
GII87_RS07105	1352670..1353620	+	951	WP_003245086.1	ring-cleaving dioxygenase	-
GII87_RS07110	1353721..1353867	+	147	WP_003244977.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin SpoIISA, type II toxin-antitoxin system (3-238)

Antitoxin

• Antitoxin SpoIISB (1-56)

Sequences

Toxin

Protein

• Length: 249 a.a.
• Molecular weight: 29061.50 Da
• Isoelectric Point: 4.6191

>T143003 WP_003244695.1 NZ_CP045922:c1348948-1348202 [Bacillus subtilis]
MVLFFQIMVWCIVAGLGLYVYATWRFEAKVKEKMSAIRKTWYLLFVLGAMVYWTYEPTSLFTHWERYLIVAVSFALIDAF
IFLSAYVKKLAGSELETDTREILEENNEMLHMYLNRLKTYQYLLKNEPIHVYYGSIDAYAEGIDKLLKTYADKMNLTASL
CHYSTQADKDRLTEHMDDPADVQTRLDRKDVYYDQYGKVVLIPFTIETQNYVIKLTSDSIVTEFDYLLFTSLTSIYDLVL
PIEEEGEG

Nucleotide

• Length: 747 bp

>T143003 NZ_CP060924:1081490-1081825 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6654.72 Da
• Isoelectric Point: 11.0203

>AT143003 WP_003232646.1 NZ_CP045922:c1348202-1348032 [Bacillus subtilis]
MERAFQNRCEPRAAKPFKILKKRSTTSVASYQVSPHTARIFKENERLIDEYKRKKA

Nucleotide

• Length: 171 bp

>AT143003 NZ_CP060924:1081242-1081490 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 3O6Q

Antitoxin

• PDB: 3O6Q
• AlphaFold DB: G4EXD8