Detailed information of TA system TA142999

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 1723266..1723951
• Replicon: chromosome
• Accession: NZ_CP045920
• Organism: Pseudactinotalea sp. HY158

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GCE65_RS07715
• Protein ID: WP_153877977.1
• Coordinates: 1723266..1723700 (-)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GCE65_RS07720
• Protein ID: WP_153877978.1
• Coordinates: 1723697..1723951 (-)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCE65_RS07695	1719442..1720536	-	1095	WP_153877973.1	hypothetical protein	-
GCE65_RS07700	1720533..1721108	-	576	WP_153877974.1	RNA polymerase sigma factor	-
GCE65_RS07705	1721502..1722749	+	1248	WP_153877975.1	IS256 family transposase	-
GCE65_RS07710	1722793..1723128	+	336	WP_153877976.1	PIN domain-containing protein	-
GCE65_RS07715	1723266..1723700	-	435	WP_153877977.1	type II toxin-antitoxin system VapC family toxin	Toxin
GCE65_RS07720	1723697..1723951	-	255	WP_153877978.1	antitoxin	Antitoxin
GCE65_RS07725	1724147..1724680	+	534	WP_194928866.1	RNA polymerase sigma factor	-
GCE65_RS07730	1724677..1725750	+	1074	WP_153877980.1	hypothetical protein	-
GCE65_RS07735	1725740..1726249	-	510	WP_153877981.1	SRPBCC family protein	-
GCE65_RS07740	1726290..1726634	-	345	WP_152818104.1	carboxymuconolactone decarboxylase family protein	-
GCE65_RS07745	1726747..1728144	-	1398	WP_153877982.1	beta-glucosidase	-
GCE65_RS07750	1728151..1728771	-	621	WP_152818103.1	threonylcarbamoyl-AMP synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	1721502..1722749	1247

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (3-133)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15660.58 Da
• Isoelectric Point: 4.9134

>T142999 WP_153877977.1 NZ_CP045920:c1723700-1723266 [Pseudactinotalea sp. HY158]
VRVVDANVLLYAVNEDARNHASALEWLERALAGEDTVGFGWIAIVAFVRLSTRPGIFSAPLSSAEAFAQVDDWISAPGGR
ILHPGERHLGILSSLLDELGTAGNLVNDAHLAALAVEHRASIVSYDNDSSRFDVRRYTPEQLLR

Nucleotide

• Length: 435 bp

>T142999 NZ_CP060924:309046-309321 [Escherichia coli]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATAGTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGCAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9235.62 Da
• Isoelectric Point: 11.5606

>AT142999 WP_153877978.1 NZ_CP045920:c1723951-1723697 [Pseudactinotalea sp. HY158]
MRTTVTLDADTEALVRRLMAERGVSFKRALNDAIRAGDPSKAPKRGRITRTRRMGAAAVDLDRALSLAGTLEDDELIRRL
QVGT

Nucleotide

• Length: 255 bp

>AT142999 NZ_CP060924:309318-309677 [Escherichia coli]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCAGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA