Detailed information of TA system TA142994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-Phd
• Location: 623881..624428
• Replicon: chromosome
• Accession: NZ_CP045920
• Organism: Pseudactinotalea sp. HY158

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GCE65_RS02680
• Protein ID: WP_194928796.1
• Coordinates: 624144..624428 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GCE65_RS02675
• Protein ID: WP_152817378.1
• Coordinates: 623881..624147 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GCE65_RS02660	618978..620627	-	1650	WP_153877289.1	DEAD/DEAH box helicase	-
GCE65_RS02665	620704..621983	-	1280	Protein_518	GNAT family N-acetyltransferase	-
GCE65_RS02670	622107..623789	+	1683	WP_153877290.1	DUF885 domain-containing protein	-
GCE65_RS02675	623881..624147	+	267	WP_152817378.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GCE65_RS02680	624144..624428	+	285	WP_194928796.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GCE65_RS16185	624513..624815	-	303	WP_194928797.1	hypothetical protein	-
GCE65_RS02685	624794..626767	-	1974	WP_153877291.1	helicase-associated domain-containing protein	-
GCE65_RS02690	626948..627331	+	384	WP_153877292.1	cold shock domain-containing protein	-
GCE65_RS02695	627342..628310	+	969	WP_153877293.1	DUF3027 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (33-91)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-73)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10505.00 Da
• Isoelectric Point: 9.6174

>T142994 WP_194928796.1 NZ_CP045920:624144-624428 [Pseudactinotalea sp. HY158]
VNEEEAPFELVLAPPAVRAIQQALPEAVATAVLEFATGALVRNPRRVGTPLRGELAGIYSARRGTYRVLYRINDADHDVV
VLRIEHRRDAYRAR

Nucleotide

• Length: 285 bp

>T142994 NZ_CP060919:c4422440-4422153 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTAAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGAACGCATAGAAAACGGTGAGTATCGCTGGCAGACAATCGGCC
TGGTTTACGGCATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAATCGCTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9546.67 Da
• Isoelectric Point: 5.1117

>AT142994 WP_152817378.1 NZ_CP045920:623881-624147 [Pseudactinotalea sp. HY158]
MSTGPLRDIRNRFSDVVDRVQHEHERVTVTRNGRPAAVIISPDDLAALEETLAVLSDPQALADIREADAAYAAGEVVRGA
DAARALRR

Nucleotide

• Length: 267 bp

>AT142994 NZ_CP060919:c4422166-4421864 [Escherichia coli]
ATGAGCATGGTTAAACATAAACGAGGGACCTCCTCTACACTCAATGTGCAGCATGAGGCCGAACTGAAGGCGCTGGCGAA
TAAATCCGACGAAGATATTGATTACAGTGATATCCCACCTTCCAGCGATGAGCAATGGTCTAACGCAGAACGCGGTAAGT
TTTATCGGCCATTGAAAACTCAGGCTTCGGTGCGTATTGATGCTGATGTGATGGAATGGTTGAAGCGACCAGGTAAAGGC
TATCAAACTCGCCTGAACGCAATTTTACGTGAAGCGATGCTGCGCGATCAGAACAAAAAATAA