142991

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tad-ata/COG5606-COG4679
Location 213160..213853 Replicon plasmid pCF39S
Accession NZ_CP045917
Organism Pseudomonas aeruginosa strain CF39S

Toxin (Protein)


Gene name tad Uniprot ID N2IHR9
Locus tag GIY05_RS32715 Protein ID WP_003151133.1
Coordinates 213476..213853 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name ata Uniprot ID N2IIN5
Locus tag GIY05_RS32710 Protein ID WP_001172026.1
Coordinates 213160..213495 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIY05_RS32680 208401..210785 - 2385 WP_141989052.1 penicillin acylase family protein -
GIY05_RS32685 210919..211950 + 1032 WP_141989050.1 AraC family transcriptional regulator -
GIY05_RS32690 211947..212057 - 111 Protein_202 thiol-disulfide isomerase -
GIY05_RS32695 212092..212463 - 372 WP_023294894.1 hypothetical protein -
GIY05_RS32700 212460..212786 - 327 WP_000091614.1 hypothetical protein -
GIY05_RS32705 212810..213145 - 336 WP_000741275.1 hypothetical protein -
GIY05_RS32710 213160..213495 - 336 WP_001172026.1 helix-turn-helix domain-containing protein Antitoxin
GIY05_RS32715 213476..213853 - 378 WP_003151133.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GIY05_RS32720 214045..214647 + 603 WP_010465829.1 recombinase family protein -
GIY05_RS32725 214631..217660 + 3030 WP_010799689.1 Tn3 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(6)-Id / aph(3'')-Ib / sul1 / tet(G) / floR - 1..468631 468631


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(16-109)

Antitoxin

Cro/C1-type helix-turn-helix domain

(40-93)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13705.78 Da        Isoelectric Point: 9.4693

>T142991 WP_003151133.1 NZ_CP045917:c213853-213476 [Pseudomonas aeruginosa]
MTNKEKPLEWIASSHKDLMALPSDVRRRFGYALSLAQIGDQDDAAKVLKGFGGAGVLEVVEDDAGGTYRAVYTVKFAEAV
FVLHCFQKKSKSGIATPKADMDIIRARLKVAEVLAQELRNAKTNH

Download         Length: 378 bp

>T142991 NZ_CP060919:3771711-3771929 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12201.39 Da        Isoelectric Point: 10.6058

>AT142991 WP_001172026.1 NZ_CP045917:c213495-213160 [Pseudomonas aeruginosa]
MQKRIIEGVEVQRSSGNVFADLGLPDAEKLKIKTGLVVEIRRAMRALGLTQQAAAKRMGIPQPKVSGMMRGDFTNLSERK
LMDCLNRLGYDIEIKVRPAAEPIGHLTLATA

Download         Length: 336 bp

>AT142991 NZ_CP060919:3771311-3771685 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A024ELN5

Antitoxin

Source ID Structure
AlphaFold DB A0A024EKI7

References