Detailed information of TA system TA142987

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 5498116..5498756
• Replicon: chromosome
• Accession: NZ_CP045916
• Organism: Pseudomonas aeruginosa strain CF39S

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GIY05_RS25670
• Protein ID: WP_003105740.1
• Coordinates: 5498116..5498526 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: Q6X2S2
• Locus tag: GIY05_RS25675
• Protein ID: WP_003158175.1
• Coordinates: 5498526..5498756 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY05_RS25630	5493958..5494155	+	198	WP_003105756.1	hypothetical protein	-
GIY05_RS25635	5494311..5495039	+	729	WP_003105754.1	TIGR03761 family integrating conjugative element protein	-
GIY05_RS25640	5495045..5495593	+	549	WP_003105753.1	DUF3158 family protein	-
GIY05_RS25645	5495640..5496479	+	840	WP_003105750.1	Rha family transcriptional regulator	-
GIY05_RS25650	5496509..5496997	+	489	WP_003105748.1	single-stranded DNA-binding protein	-
GIY05_RS25655	5497105..5497350	+	246	WP_023102333.1	CrpP family protein	-
GIY05_RS25660	5497416..5497634	-	219	WP_003105747.1	hypothetical protein	-
GIY05_RS25665	5497834..5498094	-	261	WP_003105742.1	hypothetical protein	-
GIY05_RS25670	5498116..5498526	-	411	WP_003105740.1	tRNA(fMet)-specific endonuclease VapC	Toxin
GIY05_RS25675	5498526..5498756	-	231	WP_003158175.1	antitoxin	Antitoxin
GIY05_RS25680	5499012..5500931	+	1920	WP_004352838.1	type I DNA topoisomerase	-
GIY05_RS25685	5501239..5501448	+	210	WP_003105733.1	cold-shock protein	-
GIY05_RS25690	5501669..5503558	+	1890	WP_003105732.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5478545..5581842	103297

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (7-35)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 15450.81 Da
• Isoelectric Point: 7.3233

>T142987 WP_003105740.1 NZ_CP045916:c5498526-5498116 [Pseudomonas aeruginosa]
MLKFMLDTNICIFTIKNRPQEVREAFLRHHDQMCISTVTLMELIYGAEKSSNPSRNLADVEGFAARLEVLKYDQEAAAHT
GQLRAELARLGKQIGPYDQMIAGHARSQGLIVVTNNRREFDRVPGLRVEDWVVPIV

Nucleotide

• Length: 411 bp

>T142987 NZ_CP060919:c2786118-2785942 [Escherichia coli]
GTGAAACAAAGCGAGTTCAGACGTTGGCTCGAATCTCAGGGCGTCGATGTAGCGAATGGCAGCAACCATTTGAAACTCAG
GTTTCATGGGAGGCGCAGTGTCATGCCGCGTCACCCCTGCGATGAGATTAAAGAACCATTGCGTAAAGCAATCCTGAAAC
AACTCGGTTTGAGTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8480.41 Da
• Isoelectric Point: 4.2821

>AT142987 WP_003158175.1 NZ_CP045916:c5498756-5498526 [Pseudomonas aeruginosa]
MEQGSVFQSNRSQAVRLPKAVALPDDVKRVDVVVVGRARIITPAGESWDSWFDGEGVTADFMAEREQPADQERETF

Nucleotide

• Length: 231 bp

>AT142987 NZ_CP060919:c2785896-2785480 [Escherichia coli]
ATGCGTTATCCCGTCACTCTTACACCCGCGCCGGAAGGCGGTTATATGGTTTCTTTTGTGGATATCCCTGAAGCGTTGAC
GCAGGGCGAAACTGTCGCTGAAGCGATGGAAGCGGCAAAAGATGCTTTACTGACCGCATTTGATTTTTATTTTGAAGATA
ACGAGCTTATCCCTTTACCTTCGCCATTAAATAGTCATGATCACTTTATTGAAGTACCTTTGAGCGTCGCCTCTAAGGTA
TTGCTGTTAAATGCTTTTTTACAGTCAGAAATCACTCAGCAAGAGTTAGCCAGGCGAATTGGCAAACCTAAACAGGAGAT
TACTCGCCTATTTAACTTGCATCATGCGACAAAAATCGACGCCGTCCAGCTCGCGGCAAAGGCGCTTGGCAAAGAGTTAT
CGCTGGTGATGGTTTAA

Structures

Antitoxin

• AlphaFold DB: Q6X2S2