Detailed information of TA system TA142985

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/-
• Location: 3806107..3807328
• Replicon: chromosome
• Accession: NZ_CP045916
• Organism: Pseudomonas aeruginosa strain CF39S

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: A0A0C7CS07
• Locus tag: GIY05_RS17660
• Protein ID: WP_003102740.1
• Coordinates: 3806750..3807328 (+)
• Length: 193 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GIY05_RS17655
• Protein ID: WP_003088112.1
• Coordinates: 3806107..3806661 (+)
• Length: 185 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY05_RS17630	3801675..3802226	+	552	WP_003102727.1	XRE family transcriptional regulator	-
GIY05_RS17635	3802223..3802621	-	399	WP_003102731.1	NADH-quinone oxidoreductase subunit A	-
GIY05_RS17640	3802634..3802957	-	324	WP_003102734.1	multidrug efflux SMR transporter	-
GIY05_RS17645	3803288..3803749	+	462	WP_003102736.1	(2Fe-2S)-binding protein	-
GIY05_RS17650	3803754..3805949	+	2196	WP_003102738.1	xanthine dehydrogenase family protein molybdopterin-binding subunit	-
GIY05_RS17655	3806107..3806661	+	555	WP_003088112.1	helix-turn-helix domain-containing protein	Antitoxin
GIY05_RS17660	3806750..3807328	+	579	WP_003102740.1	HD domain-containing protein	Toxin
GIY05_RS17665	3807438..3808625	-	1188	WP_003102741.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GIY05_RS17670	3808615..3810786	-	2172	WP_003088105.1	type I secretion system permease/ATPase	-
GIY05_RS17675	3810776..3812053	-	1278	WP_003102743.1	TolC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HD domain (16-170)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-62)

Sequences

Toxin

Protein

• Length: 193 a.a.
• Molecular weight: 21869.70 Da
• Isoelectric Point: 5.3655

>T142985 WP_003102740.1 NZ_CP045916:3806750-3807328 [Pseudomonas aeruginosa]
MSLELLSNRLEFLREAERLKDVLRSAHTSSGRPESTAEHSWRLCLMALAFEDQLAGLDLGKVLRMCVVHDLGEAIHGDIP
AVEQAAHPDKGEQERADLLQLTRHLDAPLRDRLLALWDEYERGESAEALAVKALDKLETLLQHTQGDNPADFDYAFNLDY
GRRYTDRAPLFKTLRELLDARTRQRLAARRDA

Nucleotide

• Length: 579 bp

>T142985 NZ_CP060919:2337918-2338021 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 185 a.a.
• Molecular weight: 20454.63 Da
• Isoelectric Point: 8.1590

>AT142985 WP_003088112.1 NZ_CP045916:3806107-3806661 [Pseudomonas aeruginosa]
MTEPLPSSSLGPALRRWRLLHRVKQTHAAELLGVAQSTISRWESGTQALLVEERARLERLLGARLEAAADQALARLVEAN
PQPVHLICDLTHRLLACSPARAAQFGVPLGELLDRSLWPYCSEEILRQEATLEELGWRELLAPPALEFASGANASAIVPI
RRSRCRWTRMTLSDGRAVRLVETL

Nucleotide

• Length: 555 bp

>AT142985 NZ_CP060919:c2338059-2337788 [Escherichia coli]
AAAGTCAGCGAAGGAAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGTGGTGGTCATCAGCTGAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Structures

Toxin

• AlphaFold DB: A0A0C7CS07