142982

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) pemIK/MazF(toxin)
Location 641750..642385 Replicon chromosome
Accession NZ_CP045915
Organism Gracilibacillus sp. SCU50

Toxin (Protein)


Gene name pemK Uniprot ID A0A5Q2TGQ3
Locus tag GI584_RS03210 Protein ID WP_026287269.1
Coordinates 642035..642385 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name pemI Uniprot ID A0A5Q2TED1
Locus tag GI584_RS03205 Protein ID WP_018932004.1
Coordinates 641750..642031 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GI584_RS03190 637139..638650 + 1512 WP_153790217.1 NAD(P)H-hydrate dehydratase -
GI584_RS03195 638705..639724 + 1020 WP_153790218.1 outer membrane lipoprotein carrier protein LolA -
GI584_RS03200 640460..641578 + 1119 WP_153790219.1 alanine racemase -
GI584_RS03205 641750..642031 + 282 WP_018932004.1 hypothetical protein Antitoxin
GI584_RS03210 642035..642385 + 351 WP_026287269.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
GI584_RS03215 642553..643419 + 867 WP_153790220.1 RsbT co-antagonist protein RsbRA -
GI584_RS03220 643432..643788 + 357 WP_091485317.1 STAS domain-containing protein -
GI584_RS03225 643791..644192 + 402 WP_153790221.1 anti-sigma regulatory factor -
GI584_RS03230 644301..645305 + 1005 WP_100362056.1 PP2C family protein-serine/threonine phosphatase -
GI584_RS03235 645441..645770 + 330 WP_100362055.1 STAS domain-containing protein -
GI584_RS03240 645772..646248 + 477 WP_100362054.1 anti-sigma B factor RsbW -
GI584_RS03245 646220..647011 + 792 WP_100362053.1 RNA polymerase sigma factor SigB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(4-110)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12996.98 Da        Isoelectric Point: 5.1634

>T142982 WP_026287269.1 NZ_CP045915:642035-642385 [Gracilibacillus sp. SCU50]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVLQNDIGNRFSPTVIVAAITAQIQKAKLPTHVEIDAEKYGFERNSVILLEQI
RTIDKQRLTDKITQLDNNMMERIDRGLEISLGLTDF

Download         Length: 351 bp

>T142982 NZ_CP060919:1642982-1643380 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10621.06 Da        Isoelectric Point: 4.4508

>AT142982 WP_018932004.1 NZ_CP045915:641750-642031 [Gracilibacillus sp. SCU50]
MVLSEDLQEVAVRLPKNLLNEVDGLMKSEDSNLSDFICQATKTYLRETKKRHIQESMQRGYMEMAKINLNIASEAFQAEE
EAENTLERLVSGV

Download         Length: 282 bp

>AT142982 NZ_CP060919:1642755-1642982 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q2TGQ3

Antitoxin

Source ID Structure
AlphaFold DB A0A5Q2TED1

References