Detailed information of TA system TA142981

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 4428614..4429245
• Replicon: chromosome
• Accession: NZ_CP045913
• Organism: Serratia proteamaculans strain 336X

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: GHV41_RS21015
• Protein ID: WP_153859938.1
• Coordinates: 4428847..4429245 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: GHV41_RS21010
• Protein ID: WP_153859937.1
• Coordinates: 4428614..4428847 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHV41_RS20985	4423614..4425239	+	1626	WP_153859934.1	Tar ligand binding domain-containing protein	-
GHV41_RS20990	4425268..4426140	+	873	WP_026142427.1	protein-glutamate O-methyltransferase CheR	-
GHV41_RS20995	4426140..4427189	+	1050	WP_153859935.1	chemotaxis response regulator protein-glutamate methylesterase	-
GHV41_RS21000	4427441..4427830	+	390	WP_012145701.1	chemotaxis response regulator CheY	-
GHV41_RS21005	4427842..4428486	+	645	WP_153859936.1	protein phosphatase CheZ	-
GHV41_RS21010	4428614..4428847	+	234	WP_153859937.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GHV41_RS21015	4428847..4429245	+	399	WP_153859938.1	type II toxin-antitoxin system VapC family toxin	Toxin
GHV41_RS21020	4429374..4430525	+	1152	WP_153859939.1	flagellar type III secretion system protein FlhB	-
GHV41_RS21025	4430518..4432596	+	2079	WP_153859940.1	flagellar biosynthesis protein FlhA	-
GHV41_RS21030	4432596..4433000	+	405	WP_153859941.1	flagellar protein FlhE	-
GHV41_RS21035	4433005..4433307	-	303	WP_153859942.1	helix-turn-helix domain-containing protein	-
GHV41_RS21040	4433553..4433987	-	435	WP_153859943.1	flagellar export chaperone FlgN	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	hsiC1/vipB / hsiB1/vipA / flhD / flhC / motA / motB / cheA / cheW / cheD / cheR / cheB / cheY / cheZ / flhB / flhA / flgB / flgC / flgE / flgF / flgG / flgH / flgI / flgJ / flgK / flgL / fliR / fliQ / fliP / fliN / fliM / fliL / fliJ / fliI / fliH / fliG / fliF / fliE / fliS / fliA / fliZ / wza	4389126..4564563	175437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-125)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15017.26 Da
• Isoelectric Point: 8.1307

>T142981 WP_153859938.1 NZ_CP045913:4428847-4429245 [Serratia proteamaculans]
MFSHMLDTNIVIYVIKRRPLEVLHRFNQHAGKMVISSVTYAELIHGVEKSARPTENARVVDDFVSRLEILDYTAKAAAHY
GNIRACLERQGTPIGINDLHIAGHARSEGLVLVTNNLREFERIDGLRLQNWL

Nucleotide

• Length: 399 bp

>T142981 NZ_CP060919:1327159-1327494 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8864.80 Da
• Isoelectric Point: 4.7672

>AT142981 WP_153859937.1 NZ_CP045913:4428614-4428847 [Serratia proteamaculans]
MTLGSVFTNNRTQAVRLPAEVRFPDSVSKVSVRVVGKERILSPVENTWDSFFCTDNRVSEDFLSERADQHQSDREEF

Nucleotide

• Length: 234 bp

>AT142981 NZ_CP060919:1326911-1327159 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA