Detailed information of TA system TA142977

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH
• Location: 3340864..3341590
• Replicon: chromosome
• Accession: NZ_CP045913
• Organism: Serratia proteamaculans strain 336X

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: GHV41_RS15940
• Protein ID: WP_153859219.1
• Coordinates: 3341204..3341590 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: GHV41_RS15935
• Protein ID: WP_153859218.1
• Coordinates: 3340864..3341211 (-)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHV41_RS15915	3336809..3337327	-	519	WP_153859215.1	flavodoxin FldB	-
GHV41_RS15920	3337435..3338334	+	900	WP_153859216.1	site-specific tyrosine recombinase XerD	-
GHV41_RS15925	3338362..3339078	+	717	WP_135317816.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
GHV41_RS15930	3339085..3340818	+	1734	WP_153859217.1	single-stranded-DNA-specific exonuclease RecJ	-
GHV41_RS15935	3340864..3341211	-	348	WP_153859218.1	helix-turn-helix domain-containing protein	Antitoxin
GHV41_RS15940	3341204..3341590	-	387	WP_153859219.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GHV41_RS15945	3341846..3342944	+	1099	WP_112363199.1	peptide chain release factor 2	-
GHV41_RS15950	3342954..3344471	+	1518	WP_153859220.1	lysine--tRNA ligase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (54-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14578.59 Da
• Isoelectric Point: 8.5038

>T142977 WP_153859219.1 NZ_CP045913:c3341590-3341204 [Serratia proteamaculans]
MDIFVTDDFDKFMRKNRIFDQRICVAAQEIMHGRHDGDLGGGVFKKRLPLNQGKRGGARSVVAFKRGHHQYFVDGWLKNT
VKQTGAKEISDDELATYRELAGDFLAMSPEIIQRAIDSGYLREVKCND

Nucleotide

• Length: 387 bp

>T142977 NZ_CP060919:c833808-833344 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTTTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 13063.11 Da
• Isoelectric Point: 10.8531

>AT142977 WP_153859218.1 NZ_CP045913:c3341211-3340864 [Serratia proteamaculans]
MTDNRLLRLQKMAQRFNSIGAVSDETVRNIEARVQEREKNVRSEQAKIMDGERIKMLRERLGLSQADLAAVVNMSTTSVQ
KWERNAVKPQGSALRMLEIIEQKGVASVIISPLER

Nucleotide

• Length: 348 bp

>AT142977 NZ_CP060919:c834143-833808 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA