Detailed information of TA system TA142973

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907-MazE
• Location: 2302390..2302971
• Replicon: chromosome
• Accession: NZ_CP045913
• Organism: Serratia proteamaculans strain 336X

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: A0A5Q2VB60
• Locus tag: GHV41_RS11210
• Protein ID: WP_064645507.1
• Coordinates: 2302390..2302722 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: GHV41_RS11215
• Protein ID: WP_153858564.1
• Coordinates: 2302723..2302971 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GHV41_RS11185	2297831..2298178	+	348	WP_000631725.1	IS66 family insertion sequence element accessory protein TnpB	-
GHV41_RS11190	2298198..2299832	+	1635	WP_153857617.1	IS66 family transposase	-
GHV41_RS11195	2300044..2300220	+	177	Protein_2179	transposase domain-containing protein	-
GHV41_RS11200	2300406..2301824	-	1419	WP_153858562.1	IS4 family transposase	-
GHV41_RS11205	2301938..2302207	+	270	WP_153858563.1	hypothetical protein	-
GHV41_RS11210	2302390..2302722	-	333	WP_064645507.1	endoribonuclease MazF	Toxin
GHV41_RS11215	2302723..2302971	-	249	WP_153858564.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
GHV41_RS11220	2303175..2304149	+	975	Protein_2184	IS5 family transposase	-
GHV41_RS11225	2304440..2304577	+	138	Protein_2185	IS3 family transposase	-
GHV41_RS11230	2305045..2307729	+	2685	WP_153858565.1	autotransporter outer membrane beta-barrel domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2287571..2319706	32135
	-	inside	IScluster/Tn	-	-	2294575..2312773	18198

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (4-109)

Antitoxin

• SpoVT-AbrB domain (7-51)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11969.73 Da
• Isoelectric Point: 7.1059

>T142973 WP_064645507.1 NZ_CP045913:c2302722-2302390 [Serratia proteamaculans]
MVSRYVPNSGDLIWLDFDPVEGHEQGGHRPAVVLSPFSYNNPVGLLLCVPCTTKAKGYPFEVELSGSRESVALADQVTCV
DWRARKVTKKGTVSPEELAEIRAKAKALIG

Nucleotide

• Length: 333 bp

>T142973 NZ_CP060914:c4685411-4685238 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8843.33 Da
• Isoelectric Point: 5.7630

>AT142973 WP_153858564.1 NZ_CP045913:c2302971-2302723 [Serratia proteamaculans]
MTTVTLKKWGNSPSIRIPASVMQAAALNVEDKLDLKIDEAGQIVLIPLKSKEYSLDILLSGITPDNVHGKIDFGSPVGKE
LI

Nucleotide

• Length: 249 bp

>AT142973 NZ_CP060914:c4685735-4685439 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GATTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A5Q2VB60