Detailed information of TA system TA142938

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 55679..56418
• Replicon: chromosome
• Accession: NZ_CP045870
• Organism: Klebsiella aerogenes strain Y1

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GIY00_RS00370
• Protein ID: WP_032705770.1
• Coordinates: 55933..56418 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: GIY00_RS00365
• Protein ID: WP_015705366.1
• Coordinates: 55679..55945 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY00_RS00340	51013..51600	+	588	WP_015705371.1	hypothetical protein	-
GIY00_RS00345	51597..52917	+	1321	Protein_73	two-component sensor histidine kinase	-
GIY00_RS00350	52917..53534	+	618	WP_015705369.1	response regulator transcription factor	-
GIY00_RS00355	53630..54127	+	498	WP_032708018.1	heme-binding protein	-
GIY00_RS00360	54171..55406	-	1236	WP_020078885.1	MFS transporter	-
GIY00_RS00365	55679..55945	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
GIY00_RS00370	55933..56418	+	486	WP_032705770.1	GNAT family N-acetyltransferase	Toxin
GIY00_RS00375	56490..58106	+	1617	WP_032708017.1	FAD-NAD(P)-binding protein	-
GIY00_RS00380	58225..59325	+	1101	WP_032708016.1	NADH:flavin oxidoreductase/NADH oxidase	-
GIY00_RS00385	59382..59540	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
GIY00_RS00390	59801..60871	+	1071	WP_032708015.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17573.23 Da
• Isoelectric Point: 8.8793

>T142938 WP_032705770.1 NZ_CP045870:55933-56418 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEESHQVVGFYSLATGSVNHSEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T142938 NZ_CP060908:1843792-1844043 [Escherichia coli]
ATGCGTACAATTAGTTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT142938 WP_015705366.1 NZ_CP045870:55679-55945 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT142938 NZ_CP060908:1844040-1844294 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAGTCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAGCATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9