Detailed information of TA system TA142928

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 2859955..2860541
• Replicon: chromosome
• Accession: NZ_CP045869
• Organism: Klebsiella aerogenes strain Y3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GIY01_RS13750
• Protein ID: WP_032709318.1
• Coordinates: 2860173..2860541 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: GIY01_RS13745
• Protein ID: WP_004174006.1
• Coordinates: 2859955..2860176 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY01_RS13725	2856096..2857022	+	927	WP_015369323.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
GIY01_RS13730	2857019..2858296	+	1278	WP_015369324.1	branched chain amino acid ABC transporter permease LivM	-
GIY01_RS13735	2858293..2859060	+	768	WP_032709319.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
GIY01_RS13740	2859078..2859791	+	714	WP_086538103.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
GIY01_RS13745	2859955..2860176	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GIY01_RS13750	2860173..2860541	+	369	WP_032709318.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GIY01_RS13755	2860833..2862149	+	1317	WP_015703703.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
GIY01_RS13760	2862256..2863143	+	888	WP_015369328.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
GIY01_RS13765	2863140..2863985	+	846	WP_015369329.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
GIY01_RS13770	2863987..2865057	+	1071	WP_032709316.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2854933..2865794	10861

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Fido domain (13-86)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.97 Da
• Isoelectric Point: 9.7753

>T142928 WP_032709318.1 NZ_CP045869:2860173-2860541 [Klebsiella aerogenes]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGKLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T142928 NZ_CP060903:66407-66823 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT142928 WP_004174006.1 NZ_CP045869:2859955-2860176 [Klebsiella aerogenes]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT142928 NZ_CP060903:66180-66410 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7