Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 2810482..2811113 Replicon chromosome
Accession NZ_CP045869
Organism Klebsiella aerogenes strain Y3

Toxin (Protein)


Gene name hicA Uniprot ID A0A0H3FL31
Locus tag GIY01_RS13470 Protein ID WP_015703741.1
Coordinates 2810482..2810757 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID A0A0H3FNK6
Locus tag GIY01_RS13475 Protein ID WP_015703740.1
Coordinates 2810754..2811113 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GIY01_RS13450 2805710..2806045 + 336 WP_015703743.1 universal stress protein UspB -
GIY01_RS13455 2806111..2807607 - 1497 WP_015369272.1 inorganic phosphate transporter PitA -
GIY01_RS13460 2807838..2809031 + 1194 WP_032709350.1 NAD(P)/FAD-dependent oxidoreductase -
GIY01_RS13465 2809072..2810085 - 1014 WP_015369274.1 magnesium transporter -
GIY01_RS13470 2810482..2810757 + 276 WP_015703741.1 type II toxin-antitoxin system HicA family toxin Toxin
GIY01_RS13475 2810754..2811113 + 360 WP_015703740.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
GIY01_RS13480 2811141..2811542 - 402 WP_032709347.1 nickel-responsive transcriptional regulator NikR -
GIY01_RS13485 2811530..2812321 - 792 WP_032709345.1 nickel import ATP-binding protein NikE -
GIY01_RS13490 2812318..2813082 - 765 WP_015703737.1 nickel import ATP-binding protein NikD -
GIY01_RS13495 2813082..2813915 - 834 WP_015369278.1 nickel ABC transporter permease subunit NikC -
GIY01_RS13500 2813912..2814856 - 945 WP_015703735.1 nickel ABC transporter permease subunit NikB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10258.89 Da        Isoelectric Point: 10.4888

>T142927 WP_015703741.1 NZ_CP045869:2810482-2810757 [Klebsiella aerogenes]
MEQQVLSLRKKQRNTLEQLFKTPVPQGIKWADIESLIKALGGEIKEGRGSRCKFLLNHSIASFHRPHPSPDTDKGAVESV
RDWLITIGVKP

Download         Length: 276 bp

>T142927 NZ_CP060903:c65400-65095 [Escherichia coli]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTCCAGAGTGACATTATTGACAC
GCCTGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGTTGTCAGATAAGGTCTCCCGTGAGCTTTACCCGGTGG
TGCATGTCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAGGAAGTG
GCTGATCTCAGCCACCGAGAAAATGACATCAAAAATGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13305.02 Da        Isoelectric Point: 4.3472

>AT142927 WP_015703740.1 NZ_CP045869:2810754-2811113 [Klebsiella aerogenes]
MIKPKTPNSMEIAGQPAVINYVPELNAFRGKFLGLSGYCDFVSDSIQGLQQEGEISLQEYLADCREAGIEPYAQPEKLKT
FTLRYPESFGERLSSAAAEEQVSVNTWILETLNERLKQA

Download         Length: 360 bp

>AT142927 NZ_CP060903:c65620-65402 [Escherichia coli]
ATGAAGCAGCGCATTACAGTCACCGTCGACAGTGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGGCT
GGTAAGCACCACCCTGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGCAGGCAGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCCGACGAGAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3FL31

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3FNK6

References