Detailed information of TA system TA142925

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 14581..15320
• Replicon: chromosome
• Accession: NZ_CP045869
• Organism: Klebsiella aerogenes strain Y3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: -
• Locus tag: GIY01_RS00095
• Protein ID: WP_032705770.1
• Coordinates: 14835..15320 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: A0A0H3FSM9
• Locus tag: GIY01_RS00090
• Protein ID: WP_015705366.1
• Coordinates: 14581..14847 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GIY01_RS00065	9916..10503	+	588	WP_015705371.1	hypothetical protein	-
GIY01_RS00070	10500..11819	+	1320	WP_015705370.1	two-component sensor histidine kinase	-
GIY01_RS00075	11819..12436	+	618	WP_015705369.1	response regulator transcription factor	-
GIY01_RS00080	12532..13029	+	498	WP_032708018.1	heme-binding protein	-
GIY01_RS00085	13073..14308	-	1236	WP_020078885.1	MFS transporter	-
GIY01_RS00090	14581..14847	+	267	WP_015705366.1	DUF1778 domain-containing protein	Antitoxin
GIY01_RS00095	14835..15320	+	486	WP_032705770.1	GNAT family N-acetyltransferase	Toxin
GIY01_RS00100	15392..17008	+	1617	WP_032708017.1	FAD-NAD(P)-binding protein	-
GIY01_RS00105	17127..18227	+	1101	WP_032708016.1	NADH:flavin oxidoreductase/NADH oxidase	-
GIY01_RS00110	18284..18442	-	159	WP_002903230.1	YqaE/Pmp3 family membrane protein	-
GIY01_RS00115	18703..19773	+	1071	WP_032708015.1	mannonate dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (27-151)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17573.23 Da
• Isoelectric Point: 8.8793

>T142925 WP_032705770.1 NZ_CP045869:14835-15320 [Klebsiella aerogenes]
MGKISAPAPLSSHHQIAEFCCGETVLDQWLKQRGLKNQAQGAARTFVVCKEESHQVVGFYSLATGSVNHSEATGGLRRNM
PDPIPVIILARLAIDCAFHGQGLGADLLHDALLRSYRVAENVGVRALMVHALTDSAKRFYLHHGFKASTTQERTLFLALP
K

Nucleotide

• Length: 486 bp

>T142925 NZ_CP060902:c4365710-4365537 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10085.56 Da
• Isoelectric Point: 4.6558

>AT142925 WP_015705366.1 NZ_CP045869:14581-14847 [Klebsiella aerogenes]
MKSDIQLNIRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNLSDEQYAEFIDMLDAPVEANPALDKLL
ARKPQWEK

Nucleotide

• Length: 267 bp

>AT142925 NZ_CP060902:c4366034-4365738 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0H3FSM9